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Exceptionally slow kinetics of the intramolecular quadruplex formed by the Oxytricha telomeric repeat

Exceptionally slow kinetics of the intramolecular quadruplex formed by the Oxytricha telomeric repeat
Exceptionally slow kinetics of the intramolecular quadruplex formed by the Oxytricha telomeric repeat
We examined the stability and kinetics of folding of the Oxytricha telomeric repeat sequence (G4T4)4. Fluorescence melting experiments show that this intramolecular quadruplex, which is more stable in potassium- than sodium-containing buffers, shows considerable hysteresis between the melting and annealing profiles, even when heated at a rate of 0.05 °C min–1. Quantitative analysis of this hysteresis, together with temperature-jump relaxation experiments show that the dissociation is exceptionally slow with a half-life of about 10 years at 37 °C in the presence of 50 mM K+. The association reaction has a half-life of a few seconds at 37 °C, but becomes slower at elevated temperatures consistent with the suggestion that association occurs by a nucleation-zipper mechanism.
dna quadruplexes, g-quartet, crystal-structure, nucleic-acids, g-tetraplex, stability, potassium, loop, oligonucleotides, parallel
1477-0520
4153-4157
Brown, Nicholas M.
4f7ebb64-a704-4289-acb1-8ae0cc2f9ac3
Rachwal, Phillip A.
8bd3be31-babc-456b-840e-6447b57c6999
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Brown, Nicholas M.
4f7ebb64-a704-4289-acb1-8ae0cc2f9ac3
Rachwal, Phillip A.
8bd3be31-babc-456b-840e-6447b57c6999
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f

Brown, Nicholas M., Rachwal, Phillip A., Brown, Tom and Fox, Keith R. (2005) Exceptionally slow kinetics of the intramolecular quadruplex formed by the Oxytricha telomeric repeat. Organic & Biomolecular Chemistry, 3 (22), 4153-4157. (doi:10.1039/b511706b). (PMID:16267597)

Record type: Article

Abstract

We examined the stability and kinetics of folding of the Oxytricha telomeric repeat sequence (G4T4)4. Fluorescence melting experiments show that this intramolecular quadruplex, which is more stable in potassium- than sodium-containing buffers, shows considerable hysteresis between the melting and annealing profiles, even when heated at a rate of 0.05 °C min–1. Quantitative analysis of this hysteresis, together with temperature-jump relaxation experiments show that the dissociation is exceptionally slow with a half-life of about 10 years at 37 °C in the presence of 50 mM K+. The association reaction has a half-life of a few seconds at 37 °C, but becomes slower at elevated temperatures consistent with the suggestion that association occurs by a nucleation-zipper mechanism.

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More information

e-pub ahead of print date: 20 October 2005
Published date: 21 November 2005
Keywords: dna quadruplexes, g-quartet, crystal-structure, nucleic-acids, g-tetraplex, stability, potassium, loop, oligonucleotides, parallel
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 20746
URI: https://eprints.soton.ac.uk/id/eprint/20746
ISSN: 1477-0520
PURE UUID: 11f60dc3-543d-404e-80b9-346e8c2a6cc4
ORCID for Keith R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

Catalogue record

Date deposited: 02 Mar 2006
Last modified: 06 Jun 2018 13:16

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