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Simultaneous high speed optical and impedance analysis of single particles with a microfluidic cytometer

Simultaneous high speed optical and impedance analysis of single particles with a microfluidic cytometer
Simultaneous high speed optical and impedance analysis of single particles with a microfluidic cytometer
We describe a microfluidic cytometer that performs simultaneous optical and electrical characterisation of particles. The microfluidic chip measures side scattered light, signal extinction and fluorescence using integrated optical fibres coupled to photomultiplier tubes. The channel is 80 mm high and 200 mm wide, and made from SU-8 patterned and sandwiched between glass substrates. Particles were focused into the analysis region using 1-D hydrodynamic focusing and typical particle velocities were 0.1 ms?1. Excitation light is coupled into the detection channel with an optical fibre and focused into the channel using an integrated compound air lens. The electrical impedance of particles is measured at 1 MHz using micro-electrodes fabricated on the channel top and bottom. This data is used to accurately size the particles. The system is characterised using a range of different sized polystyrene beads (fluorescent and non-fluorescent). Single and mixed populations of beads were measured and the data compared with a conventional flow cytometer.
1473-0197
118-126
Barat, David
c7bf457e-3aef-48af-85ed-dfcee368ca76
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Benazzi, Giuseppe
0a1faa41-fbc8-426b-a942-f35d44757ebd
Mowlem, Matthew Charles
6f633ca2-298f-48ee-a025-ce52dd62124f
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Barat, David
c7bf457e-3aef-48af-85ed-dfcee368ca76
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Benazzi, Giuseppe
0a1faa41-fbc8-426b-a942-f35d44757ebd
Mowlem, Matthew Charles
6f633ca2-298f-48ee-a025-ce52dd62124f
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Barat, David, Spencer, Daniel, Benazzi, Giuseppe, Mowlem, Matthew Charles and Morgan, Hywel (2012) Simultaneous high speed optical and impedance analysis of single particles with a microfluidic cytometer. Lab on a Chip, 12 (1), 118-126.

Record type: Article

Abstract

We describe a microfluidic cytometer that performs simultaneous optical and electrical characterisation of particles. The microfluidic chip measures side scattered light, signal extinction and fluorescence using integrated optical fibres coupled to photomultiplier tubes. The channel is 80 mm high and 200 mm wide, and made from SU-8 patterned and sandwiched between glass substrates. Particles were focused into the analysis region using 1-D hydrodynamic focusing and typical particle velocities were 0.1 ms?1. Excitation light is coupled into the detection channel with an optical fibre and focused into the channel using an integrated compound air lens. The electrical impedance of particles is measured at 1 MHz using micro-electrodes fabricated on the channel top and bottom. This data is used to accurately size the particles. The system is characterised using a range of different sized polystyrene beads (fluorescent and non-fluorescent). Single and mixed populations of beads were measured and the data compared with a conventional flow cytometer.

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More information

Published date: 2012
Organisations: Electronics & Computer Science, Ocean Technology and Engineering

Identifiers

Local EPrints ID: 207499
URI: https://eprints.soton.ac.uk/id/eprint/207499
ISSN: 1473-0197
PURE UUID: 17db16ad-898d-43a3-861e-c2c912265b25
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 10 Jan 2012 10:47
Last modified: 11 Nov 2017 00:10

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Contributors

Author: David Barat
Author: Daniel Spencer
Author: Giuseppe Benazzi
Author: Hywel Morgan ORCID iD

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