Isolation, differentiation and characterisation of skeletal stem cells from human bone marrow in vitro and in vivo
Isolation, differentiation and characterisation of skeletal stem cells from human bone marrow in vitro and in vivo
In this chapter, we describe techniques for the isolation and characterization of skeletal stem cells from human bone marrow. The methods for enrichment of STRO-1 positive cells using magnetic activated cell sorting are described and we also cover techniques for establishing and characterising osteogenic, adipogenic, and chondrogenic cultures from these cells. Finally, we present methods for studying the ability of these cells to produce bone in vivo using diffusion chambers which have been implanted subcutaneously in mice
978-1-61779-414-8
83-99
Tare, Rahul S.
587c9db4-e409-4e7c-a02a-677547ab724a
Mitchell, Peter D.
a6de2a77-02bc-4679-8717-4ad1153464bd
Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
January 2012
Tare, Rahul S.
587c9db4-e409-4e7c-a02a-677547ab724a
Mitchell, Peter D.
a6de2a77-02bc-4679-8717-4ad1153464bd
Kanczler, Janos M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Tare, Rahul S., Mitchell, Peter D., Kanczler, Janos M. and Oreffo, Richard O.C.
(2012)
Isolation, differentiation and characterisation of skeletal stem cells from human bone marrow in vitro and in vivo.
In,
Helfrich, Miep H. H. and Ralston, Stuart H. H.
(eds.)
Bone Research Protocols.
(Methods in Molecular Biology, 816, 816)
New York, US.
Springer, .
(doi:10.1007/978-1-61779-415-5_7).
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Book Section
Abstract
In this chapter, we describe techniques for the isolation and characterization of skeletal stem cells from human bone marrow. The methods for enrichment of STRO-1 positive cells using magnetic activated cell sorting are described and we also cover techniques for establishing and characterising osteogenic, adipogenic, and chondrogenic cultures from these cells. Finally, we present methods for studying the ability of these cells to produce bone in vivo using diffusion chambers which have been implanted subcutaneously in mice
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Published date: January 2012
Organisations:
Human Development & Health
Identifiers
Local EPrints ID: 207755
URI: http://eprints.soton.ac.uk/id/eprint/207755
ISBN: 978-1-61779-414-8
PURE UUID: b90cc0ac-5193-4d85-a389-3ac661010962
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Date deposited: 13 Jan 2012 14:24
Last modified: 11 Jul 2024 01:43
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Contributors
Author:
Peter D. Mitchell
Author:
Janos M. Kanczler
Editor:
Miep H. H. Helfrich
Editor:
Stuart H. H. Ralston
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