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Scanning electrochemical microscopy (SECM): localized glucose oxidase immobilization via the direct electrochemical microspotting of polypyrrole-biotin films

Scanning electrochemical microscopy (SECM): localized glucose oxidase immobilization via the direct electrochemical microspotting of polypyrrole-biotin films
Scanning electrochemical microscopy (SECM): localized glucose oxidase immobilization via the direct electrochemical microspotting of polypyrrole-biotin films
This paper describes the successful combination of nanostructured microelectrodes, biotin-avidin chemistry and the direct and generation-collection modes of the scanning electrochemical microscope (SECM) to enable the fabrication, functionalization and characterization of biologically active microspots. The SECM tip was used as an electrochemical "pen" to drive the deposition of a micrometre size spot of biotinylated polypyrrole. Subsequent reaction with avidin and a biotinylated enzyme enabled the construction of a "molecular sandwich" capable of producing H2O2. The SECM tip was then used to "read" the activity of the microspot. A major contribution to this work was the use of mesoporous platinum tips to reliably detect the localized production Of H2O2 contrast to previous approaches this combination of localized deposition, high selectivity of the biotin-avidin binding and reliable imaging only requires one instrument and offers a valuable alternative for the fabrication and characterization of novel multi-analyte biosensor arrays.
secm, mesoporous platinum microdiscs, biotinylated pyrrole, biomolecule immobilization, generation collection, direct modehigh-resolution deposition, enzyme ultramicroelectrodes, electrodesurfaces, hydrogen-peroxide, polymerization, dna, microelectrodes, construction
1388-2481
135-140
Evans, Stuart A.G.
0fdae783-b228-45b6-b66c-54ad333c017e
Brakha, Karina
a80d0950-123e-4e88-98ff-41ad5b78256d
Billon, Martial
ae30652d-af52-4f4a-ba27-6b06845cf65f
Mailley, Pascal
f9c8b6b4-5298-4520-ad4a-2c0fbad64905
Denuault, Guy
5c76e69f-e04e-4be5-83c5-e729887ffd4e
Evans, Stuart A.G.
0fdae783-b228-45b6-b66c-54ad333c017e
Brakha, Karina
a80d0950-123e-4e88-98ff-41ad5b78256d
Billon, Martial
ae30652d-af52-4f4a-ba27-6b06845cf65f
Mailley, Pascal
f9c8b6b4-5298-4520-ad4a-2c0fbad64905
Denuault, Guy
5c76e69f-e04e-4be5-83c5-e729887ffd4e

Evans, Stuart A.G., Brakha, Karina, Billon, Martial, Mailley, Pascal and Denuault, Guy (2005) Scanning electrochemical microscopy (SECM): localized glucose oxidase immobilization via the direct electrochemical microspotting of polypyrrole-biotin films. Electrochemistry Communications, 7 (2), 135-140. (doi:10.1016/j.elecom.2004.11.019).

Record type: Article

Abstract

This paper describes the successful combination of nanostructured microelectrodes, biotin-avidin chemistry and the direct and generation-collection modes of the scanning electrochemical microscope (SECM) to enable the fabrication, functionalization and characterization of biologically active microspots. The SECM tip was used as an electrochemical "pen" to drive the deposition of a micrometre size spot of biotinylated polypyrrole. Subsequent reaction with avidin and a biotinylated enzyme enabled the construction of a "molecular sandwich" capable of producing H2O2. The SECM tip was then used to "read" the activity of the microspot. A major contribution to this work was the use of mesoporous platinum tips to reliably detect the localized production Of H2O2 contrast to previous approaches this combination of localized deposition, high selectivity of the biotin-avidin binding and reliable imaging only requires one instrument and offers a valuable alternative for the fabrication and characterization of novel multi-analyte biosensor arrays.

Full text not available from this repository.

More information

Published date: 1 February 2005
Keywords: secm, mesoporous platinum microdiscs, biotinylated pyrrole, biomolecule immobilization, generation collection, direct modehigh-resolution deposition, enzyme ultramicroelectrodes, electrodesurfaces, hydrogen-peroxide, polymerization, dna, microelectrodes, construction

Identifiers

Local EPrints ID: 20792
URI: http://eprints.soton.ac.uk/id/eprint/20792
ISSN: 1388-2481
PURE UUID: d1f321b8-d19d-4329-b974-273b8227c614
ORCID for Guy Denuault: ORCID iD orcid.org/0000-0002-8630-9492

Catalogue record

Date deposited: 03 Mar 2006
Last modified: 07 Oct 2020 01:36

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