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Retrieval from the ER-golgi intermediate compartment is key to the targeting of c-terminally anchored ER-resident proteins

Butler, John, Watson, Helen R., Lee, Anthony G., Schuppe, Hans-Jurgen and East, J. Malcolm (2011) Retrieval from the ER-golgi intermediate compartment is key to the targeting of c-terminally anchored ER-resident proteins Journal of Cellular Biochemistry, 112, (12), pp. 3543-3548. (doi:10.1002/jcb.23281).

Record type: Article

Abstract

Endoplasmic reticulum (ER) resident proteins may be maintained in the ER by retention, where the leak into post-ER compartments is absent or slow, or retrieval, where a significant leak is countered by retrieval from post-ER compartments. Here the targeting of the C-terminally anchored protein ER-resident protein, cytochrome b5a (cytb5a), considered to be maintained in the ER mainly by the process of retention, is compared with that of sarcolipin (SLN) and phospholamban (PLB); also C-terminally anchored ER-residents. Laser confocal microscopy, and cell fractionation of green fluorescent protein-tagged constructs expressed in COS 7 cells indicate that while calnexin appears to be retained in the ER with no evidence of leak into the ER–Golgi intermediate compartment (ERGIC), significant amounts of cytb5a, SLN, and PLB are detectable in the ERGIC, indicating that there is considerable leak from the ER. This is supported by an in vitro budding assay that shows that while small amounts of calnexin appear in the transport vesicles budding off from the ER, significant amounts of cytb5a and SLN are found in such vesicles. These data support the hypothesis that retrieval plays a major role in ensuring that C-terminally anchored proteins are maintained in the ER

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More information

e-pub ahead of print date: 22 October 2011
Published date: December 2011
Keywords: calnexin, cytochrome b5, phospholamban, retention, retrieval, targeting
Organisations: Molecular and Cellular

Identifiers

Local EPrints ID: 208149
URI: http://eprints.soton.ac.uk/id/eprint/208149
ISSN: 0730-2312
PURE UUID: cad76513-a548-49a8-bd73-37a2a30832da

Catalogue record

Date deposited: 19 Jan 2012 15:26
Last modified: 18 Jul 2017 10:49

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Contributors

Author: John Butler
Author: Helen R. Watson
Author: Anthony G. Lee
Author: Hans-Jurgen Schuppe
Author: J. Malcolm East

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