A simple setup for the study of microvolume frozen samples using Raman spectroscopy
A simple setup for the study of microvolume frozen samples using Raman spectroscopy
A method for obtaining resonance Raman spectra from microvolume (2-5 mu l) frozen samples is described. The procedure involves trapping and freezing a microdroplet in a specially designed sample holder using a cryostream. The method makes it possible to prepare numerous low-volume air-sensitive samples in a suitable environment for spectroscopic studies. We have demonstrated the potential of the setup measuring the resonance Raman spectra of 4-thiouracil and horse heart myoglobin.
resonance raman, vibrational-modes, heme-proteins, myoglobin, spectra, ferredoxin, crystals, binding, spin
Kriek, Marco
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Neylon, Cameron
697f067b-db25-4c41-9618-28f4b74f73aa
Roach, Peter L.
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Clark, Ian P.
dc95c211-3a56-4ebb-ba71-9941bf34a253
Parker, Anthony W.
9b5a0329-1ffe-4595-b330-861677c0e9f0
1 October 2005
Kriek, Marco
c3e820c2-c59b-49c8-a792-f64a69e4401a
Neylon, Cameron
697f067b-db25-4c41-9618-28f4b74f73aa
Roach, Peter L.
50f9fc91-e333-4be6-9634-6bb6b4415753
Clark, Ian P.
dc95c211-3a56-4ebb-ba71-9941bf34a253
Parker, Anthony W.
9b5a0329-1ffe-4595-b330-861677c0e9f0
Kriek, Marco, Neylon, Cameron, Roach, Peter L., Clark, Ian P. and Parker, Anthony W.
(2005)
A simple setup for the study of microvolume frozen samples using Raman spectroscopy.
Review of Scientific Instruments, 76 (10).
(doi:10.1063/1.2054486).
Abstract
A method for obtaining resonance Raman spectra from microvolume (2-5 mu l) frozen samples is described. The procedure involves trapping and freezing a microdroplet in a specially designed sample holder using a cryostream. The method makes it possible to prepare numerous low-volume air-sensitive samples in a suitable environment for spectroscopic studies. We have demonstrated the potential of the setup measuring the resonance Raman spectra of 4-thiouracil and horse heart myoglobin.
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Published date: 1 October 2005
Keywords:
resonance raman, vibrational-modes, heme-proteins, myoglobin, spectra, ferredoxin, crystals, binding, spin
Identifiers
Local EPrints ID: 20840
URI: http://eprints.soton.ac.uk/id/eprint/20840
ISSN: 0034-6748
PURE UUID: a04701c9-61de-4350-805c-b585ffaa4a76
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Date deposited: 02 Mar 2006
Last modified: 15 Mar 2024 06:26
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Contributors
Author:
Marco Kriek
Author:
Cameron Neylon
Author:
Peter L. Roach
Author:
Ian P. Clark
Author:
Anthony W. Parker
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