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A simple setup for the study of microvolume frozen samples using Raman spectroscopy

A simple setup for the study of microvolume frozen samples using Raman spectroscopy
A simple setup for the study of microvolume frozen samples using Raman spectroscopy
A method for obtaining resonance Raman spectra from microvolume (2-5 mu l) frozen samples is described. The procedure involves trapping and freezing a microdroplet in a specially designed sample holder using a cryostream. The method makes it possible to prepare numerous low-volume air-sensitive samples in a suitable environment for spectroscopic studies. We have demonstrated the potential of the setup measuring the resonance Raman spectra of 4-thiouracil and horse heart myoglobin.
resonance raman, vibrational-modes, heme-proteins, myoglobin, spectra, ferredoxin, crystals, binding, spin
0034-6748
Kriek, Marco
c3e820c2-c59b-49c8-a792-f64a69e4401a
Neylon, Cameron
697f067b-db25-4c41-9618-28f4b74f73aa
Roach, Peter L.
50f9fc91-e333-4be6-9634-6bb6b4415753
Clark, Ian P.
dc95c211-3a56-4ebb-ba71-9941bf34a253
Parker, Anthony W.
9b5a0329-1ffe-4595-b330-861677c0e9f0
Kriek, Marco
c3e820c2-c59b-49c8-a792-f64a69e4401a
Neylon, Cameron
697f067b-db25-4c41-9618-28f4b74f73aa
Roach, Peter L.
50f9fc91-e333-4be6-9634-6bb6b4415753
Clark, Ian P.
dc95c211-3a56-4ebb-ba71-9941bf34a253
Parker, Anthony W.
9b5a0329-1ffe-4595-b330-861677c0e9f0

Kriek, Marco, Neylon, Cameron, Roach, Peter L., Clark, Ian P. and Parker, Anthony W. (2005) A simple setup for the study of microvolume frozen samples using Raman spectroscopy. Review of Scientific Instruments, 76 (10). (doi:10.1063/1.2054486).

Record type: Article

Abstract

A method for obtaining resonance Raman spectra from microvolume (2-5 mu l) frozen samples is described. The procedure involves trapping and freezing a microdroplet in a specially designed sample holder using a cryostream. The method makes it possible to prepare numerous low-volume air-sensitive samples in a suitable environment for spectroscopic studies. We have demonstrated the potential of the setup measuring the resonance Raman spectra of 4-thiouracil and horse heart myoglobin.

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More information

Published date: 1 October 2005
Keywords: resonance raman, vibrational-modes, heme-proteins, myoglobin, spectra, ferredoxin, crystals, binding, spin

Identifiers

Local EPrints ID: 20840
URI: http://eprints.soton.ac.uk/id/eprint/20840
ISSN: 0034-6748
PURE UUID: a04701c9-61de-4350-805c-b585ffaa4a76

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Date deposited: 02 Mar 2006
Last modified: 15 Jul 2019 19:25

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Contributors

Author: Marco Kriek
Author: Cameron Neylon
Author: Peter L. Roach
Author: Ian P. Clark
Author: Anthony W. Parker

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