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Interaction of legionella pneumophila and helicobacter pylori with bacterial species isolated from drinking water biofilms

Record type: Article

Background: it is well established that Legionella pneumophila is a waterborne pathogen; by contrast, the mode of Helicobacter pylori transmission remains unknown but water seems to play an important role. This work aims to study the influence of five microorganisms isolated from drinking water biofilms on the survival and integration of both of these pathogens into biofilms.

Results: firstly, both pathogens were studied for auto- and co-aggregation with the species isolated from drinking water; subsequently the formation of mono and dual-species biofilms by L. pneumophila or H. pylori with the same microorganisms was investigated. Neither auto- nor co-aggregation was observed between the microorganisms tested. For biofilm studies, sessile cells were quantified in terms of total cells by SYTO 9 staining, viable L. pneumophila or H. pylori cells were quantified using 16 S rRNA-specific peptide nucleic acid (PNA) probes and cultivable cells by standard culture techniques. Acidovorax sp. and Sphingomonas sp. appeared to have an antagonistic effect on L. pneumophila cultivability but not on the viability (as assessed by rRNA content using the PNA probe), possibly leading to the formation of viable but noncultivable (VBNC) cells, whereas Mycobacterium chelonae increased the cultivability of this pathogen. The results obtained for H. pylori showed that M. chelonae and Sphingomonas sp. help this pathogen to maintain cultivability for at least 24 hours.

Conclusions: it appears that M. chelonae may have an important role in the survival of both pathogens in drinking water. This work also suggests that the presence of some microorganisms can decrease the cultivability of L. pneumophila but not the viability which indicates that the presence of autochthonous microorganisms can lead to misleading results when the safety of water is assessed by cultivable methods alone

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Citation

Gião, Maria S., Azevedo, Nuno F., Wilks, Sandra A., Vieira, Maria J. and Keevil, Charles W. (2011) Interaction of legionella pneumophila and helicobacter pylori with bacterial species isolated from drinking water biofilms BMC Microbiology, 11, (1), 57-[10pp]. (doi:10.1186/1471-2180-11-57).

More information

e-pub ahead of print date: 18 March 2011
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 209295
URI: http://eprints.soton.ac.uk/id/eprint/209295
PURE UUID: d027c93a-d6ca-4fc7-b5a0-18a0b6deedf7
ORCID for Sandra A. Wilks: ORCID iD orcid.org/0000-0002-4134-9415
ORCID for Charles W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 27 Jan 2012 14:10
Last modified: 18 Jul 2017 10:47

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Contributors

Author: Maria S. Gião
Author: Nuno F. Azevedo
Author: Sandra A. Wilks ORCID iD
Author: Maria J. Vieira

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