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DNA mimics for the rapid identification of microorganisms by fluorescence in situ hybridization (FISH)

Record type: Article

Fluorescence in situ hybridization (FISH) is a well-established technique that is used for a variety of purposes, ranging from pathogen detection in clinical diagnostics to the determination of chromosomal stability in stem cell research. The key step of FISH involves the detection of a nucleic acid region and as such, DNA molecules have typically been used to probe for the sequences of interest. However, since the turn of the century, an increasing number of laboratories have started to move on to the more robust DNA mimics methods, most notably peptide and locked nucleic acids (PNA and LNA). In this review, we will cover the state-of-the-art of the different DNA mimics in regard to their application as efficient markers for the presence of individual microbial cells, and consider their potential advantages and pitfalls. Available PNA probes are then reassessed in terms of sensitivity and specificity using rRNA databases. In addition, we also attempt to predict the applicability of DNA mimics in well-known techniques attempting to detect in situ low number of copies of specific nucleic acid sequences such as catalyzed reporter deposition (CARD) and recognition of individual genes (RING) FISH.

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Citation

Cerqueira, Laura, Azevedo, Nuno F., Almeida, Carina, Jardim, Tatiana, Keevil, Charles William and Vieira, Maria J. (2008) DNA mimics for the rapid identification of microorganisms by fluorescence in situ hybridization (FISH) [in special issue: Nucleic Acid Derivatives in Emerging Technologies] International Journal of Molecular Sciences, 9, (10), pp. 1944-1960. (doi:10.3390/ijms9101944). (PMID:19325728).

More information

Published date: 20 October 2008
Keywords: fish, dna mimics, pna, lna, molecular diagnostics
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 209315
URI: http://eprints.soton.ac.uk/id/eprint/209315
ISSN: 1422-0067
PURE UUID: fd809923-95f7-4a11-b1ff-c1a6e123d6d2
ORCID for Charles William Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 31 Jan 2012 12:09
Last modified: 18 Jul 2017 10:47

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Contributors

Author: Laura Cerqueira
Author: Nuno F. Azevedo
Author: Carina Almeida
Author: Tatiana Jardim
Author: Maria J. Vieira

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