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Development and application of a novel peptide nucleic acid probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula

Almeida, C., Azevedo, N.F., Iversen, C., Fanning, S., Keevil, C.W. and Vieira, M.J. (2009) Development and application of a novel peptide nucleic acid probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula Applied and Environmental Microbiology, 75, (9), pp. 2925-2930. (doi:10.1128/AEM.02470-08). (PMID:19270117).

Record type: Article

Abstract

Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants.

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More information

e-pub ahead of print date: 6 March 2009
Published date: May 2009
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 209317
URI: http://eprints.soton.ac.uk/id/eprint/209317
ISSN: 0099-2240
PURE UUID: 7ca710dd-44f1-4e9e-90b1-a98d45f7d286
ORCID for C.W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 31 Jan 2012 12:31
Last modified: 18 Jul 2017 10:47

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Contributors

Author: C. Almeida
Author: N.F. Azevedo
Author: C. Iversen
Author: S. Fanning
Author: C.W. Keevil ORCID iD
Author: M.J. Vieira

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