Almeida, C., Azevedo, N.F., Iversen, C., Fanning, S., Keevil, C.W. and Vieira, M.J.
Development and application of a novel peptide nucleic acid probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula
Applied and Environmental Microbiology, 75, (9), . (doi:10.1128/AEM.02470-08). (PMID:19270117).
Full text not available from this repository.
Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants.
Actions (login required)