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Development and application of a novel peptide nucleic acid probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula

Development and application of a novel peptide nucleic acid probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula
Development and application of a novel peptide nucleic acid probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula
Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants.
0099-2240
2925-2930
Almeida, C.
0a572983-c36b-40c3-bfc4-98e84b2bfda9
Azevedo, N.F.
c90d7c41-e45a-404d-9472-9d0b411448e7
Iversen, C.
2de07ce5-dd28-482e-a34f-8ea81dc4d829
Fanning, S.
f8d0b586-e1ca-4dac-ae4b-63228d45edec
Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Vieira, M.J.
b13909e0-b086-49fc-b589-3fb9d2a02577
Almeida, C.
0a572983-c36b-40c3-bfc4-98e84b2bfda9
Azevedo, N.F.
c90d7c41-e45a-404d-9472-9d0b411448e7
Iversen, C.
2de07ce5-dd28-482e-a34f-8ea81dc4d829
Fanning, S.
f8d0b586-e1ca-4dac-ae4b-63228d45edec
Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Vieira, M.J.
b13909e0-b086-49fc-b589-3fb9d2a02577

Almeida, C., Azevedo, N.F., Iversen, C., Fanning, S., Keevil, C.W. and Vieira, M.J. (2009) Development and application of a novel peptide nucleic acid probe for the specific detection of cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula. Applied and Environmental Microbiology, 75 (9), 2925-2930. (doi:10.1128/AEM.02470-08). (PMID:19270117)

Record type: Article

Abstract

Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants.

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e-pub ahead of print date: 6 March 2009
Published date: May 2009
Organisations: Centre for Biological Sciences
Learn more about Biological Sciences research

Identifiers

Local EPrints ID: 209317
URI: http://eprints.soton.ac.uk/id/eprint/209317
DOI: doi:10.1128/AEM.02470-08
ISSN: 0099-2240
PURE UUID: 7ca710dd-44f1-4e9e-90b1-a98d45f7d286
ORCID for C.W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

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Date deposited: 31 Jan 2012 12:31
Last modified: 15 Mar 2024 03:12

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Contributors

Author: C. Almeida
Author: N.F. Azevedo
Author: C. Iversen
Author: S. Fanning
Author: C.W. Keevil ORCID iD
Author: M.J. Vieira

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