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Time to “go large” on biofilm research: advantages of an omics approach

Time to “go large” on biofilm research: advantages of an omics approach
Time to “go large” on biofilm research: advantages of an omics approach
In nature, the biofilm mode of life is of great importance in the cell cycle for many microorganisms. Perhaps because of biofilm complexity and variability, the characterization of a given microbial system, in terms of biofilm formation potential, structure and associated physiological activity, in a large-scale, standardized and systematic manner has
been hindered by the absence of high-throughput methods. This outlook is now starting to change as new methods involving the utilization of microtiter-plates and automated spectrophotometry and microscopy systems are being developed to perform large-scale testing of microbial biofilms. Here, we evaluate if the time is ripe to start an integrated omics approach, i.e., the generation and interrogation of large datasets, to biofilms—‘‘biofomics’’. This omics approach would bring much needed insight into how biofilm formation ability is affected by a number of environmental, physiological and mutational factors and how these factors interplay between themselves in a standardized manner. This could then lead to the creation of a database where biofilm signatures are identified and
interrogated. Nevertheless, and before embarking on such an enterprise, the selection of a versatile, robust,
high-throughput biofilm growing device and of appropriate methods for biofilm analysis will have to be performed. Whether such device and analytical methods are already available, particularly for complex heterotrophic biofilms is, however, very debatable.
biofomics, high-throughput, systems biology, biofilms, database
0141-5492
477-485
Azevedo, Nuno F.
24c4eb52-0c98-443b-881f-7a1449c9ac26
Lopes, Susana P.
9bb06173-f5f1-41cf-9f91-bfe49e8a5170
Keevil, Charles W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Pereira, Maria O.
fd3f9629-fefb-444e-8714-21776c0b5f65
Vieira, Maria J.
d972e877-d85b-488c-8b0f-358f79d2fa29
Azevedo, Nuno F.
24c4eb52-0c98-443b-881f-7a1449c9ac26
Lopes, Susana P.
9bb06173-f5f1-41cf-9f91-bfe49e8a5170
Keevil, Charles W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Pereira, Maria O.
fd3f9629-fefb-444e-8714-21776c0b5f65
Vieira, Maria J.
d972e877-d85b-488c-8b0f-358f79d2fa29

Azevedo, Nuno F., Lopes, Susana P., Keevil, Charles W., Pereira, Maria O. and Vieira, Maria J. (2009) Time to “go large” on biofilm research: advantages of an omics approach. Biotechnology Letters, 31 (4), 477-485. (doi:10.1007/s10529-008-9901-4). (PMID:19116693)

Record type: Article

Abstract

In nature, the biofilm mode of life is of great importance in the cell cycle for many microorganisms. Perhaps because of biofilm complexity and variability, the characterization of a given microbial system, in terms of biofilm formation potential, structure and associated physiological activity, in a large-scale, standardized and systematic manner has
been hindered by the absence of high-throughput methods. This outlook is now starting to change as new methods involving the utilization of microtiter-plates and automated spectrophotometry and microscopy systems are being developed to perform large-scale testing of microbial biofilms. Here, we evaluate if the time is ripe to start an integrated omics approach, i.e., the generation and interrogation of large datasets, to biofilms—‘‘biofomics’’. This omics approach would bring much needed insight into how biofilm formation ability is affected by a number of environmental, physiological and mutational factors and how these factors interplay between themselves in a standardized manner. This could then lead to the creation of a database where biofilm signatures are identified and
interrogated. Nevertheless, and before embarking on such an enterprise, the selection of a versatile, robust,
high-throughput biofilm growing device and of appropriate methods for biofilm analysis will have to be performed. Whether such device and analytical methods are already available, particularly for complex heterotrophic biofilms is, however, very debatable.

Full text not available from this repository.

More information

e-pub ahead of print date: 31 December 2008
Published date: April 2009
Keywords: biofomics, high-throughput, systems biology, biofilms, database
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 209319
URI: https://eprints.soton.ac.uk/id/eprint/209319
ISSN: 0141-5492
PURE UUID: 8549726b-abf2-4c04-ba5b-ffb18d836e6e
ORCID for Charles W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 31 Jan 2012 12:39
Last modified: 06 Jun 2018 12:49

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