The University of Southampton
University of Southampton Institutional Repository

Acquisition of proteinaceous contamination through the handling of surgical instruments by hospital staff in sterile service departments

Record type: Article

Using Episcopic Differential Interference Contrast (EDIC) microscopy, this study has investigated the potential reapplication of prote ina ceouscontami napotential reapplication of proteinaceous contamination onto surgical instruments following a washer-disinfector cycle through the handling of staff within the clean room. The deposition of 0.51 ng/mm2 of protein onto surgical grade stainless steel by one finger print alone has been demonstrated. Moreover, using a previously described contamination index, a 5 to 10-fold increase in protein present on surgical instruments was noted following handling by clean-room staff under current departmental practices, relative to instruments handled by staff wearing gloves. While unlikely to pose a direct risk to patient health, subsequent sterilisation will fix protein to an instrument surface thereby decreasing the effectiveness of further decontamination cycles. Current guidelines make no recommendations surrounding the use of gloves by staff working within the clean room. However it is clear that this matter must be reviewed to limit the unnecessary transference of protein to surgical instruments.

Full text not available from this repository.

Citation

Howlin, R., Harrison, J., Secker, T. and Keevil, C.W. (2009) Acquisition of proteinaceous contamination through the handling of surgical instruments by hospital staff in sterile service departments Journal of Infection Prevention, 10, (3), pp. 106-111. (doi:10.1177/1757177409105073).

More information

Published date: May 2009
Keywords: decontamination, sterile service departments, surgical instruments, episcopic differential interference contrast/epi-fluorescence microscopy, sypro ruby
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 209637
URI: http://eprints.soton.ac.uk/id/eprint/209637
ISSN: 1876-0341
PURE UUID: 10d25902-2a6e-4f8f-902c-39c441d4344f
ORCID for T. Secker: ORCID iD orcid.org/0000-0001-7168-8592
ORCID for C.W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 31 Jan 2012 14:23
Last modified: 18 Jul 2017 10:46

Export record

Altmetrics

Contributors

Author: R. Howlin
Author: J. Harrison
Author: T. Secker ORCID iD
Author: C.W. Keevil ORCID iD

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×