Salmon consumption by pregnant women reduces ex vivo umbilical cord endothelial cell activation
Salmon consumption by pregnant women reduces ex vivo umbilical cord endothelial cell activation
Background: In vitro exposure of endothelial cells (ECs) to n−3 (omega-3) long-chain PUFAs (LCPUFAs) reduces cell adhesion molecule (CAM) expression. However, to our knowledge, no previous human studies have examined the influence of an altered diet on CAM expression.
Objective: We assessed whether salmon (rich in n−3 LCPUFAs) consumption twice a week during pregnancy affected offspring umbilical vein EC CAM expression.
Design: Women were randomly assigned to maintain their habitual diets or to consume 2 portions of salmon per week during pregnancy months 4–9. ECs were isolated from umbilical cord veins collected at birth and cultured. The cell surface expression of intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) was assessed by flow cytometry after the culture of ECs in the presence and absence of bacterial LPS for 24 h. Cytokine and growth factor concentrations in culture supernatant fluid were measured by using a multiplex assay.
Results: LPS increased the expression of VCAM-1 and the production of several cytokines and growth factors. The level of ICAM-1 expression per cell [ie, the median fluorescence intensity (MFI)] was increased by LPS stimulation in the control group (16.9 ± 2.4 compared with 135.3 ± 20.2; P < 0.001) and to a lesser extent in the salmon group (14.1 ± 3.8 compared with 65.8 ± 22.4; P = 0.037). The ICAM-1 MFI in the salmon group after LPS stimulation was lower than in the control group (P = 0.006).
Conclusion: Increased dietary salmon intake in pregnancy dampens offspring EC activation, which implicates a role for n−3 LCPUFAs in the suppression of inflammatory processes in humans. This trial was registered at clinicaltrials.gov as NCT00801502.
1418-1425
van den Elsen, L.W.
557593d4-1e12-4b8b-ad37-f7188d202d01
Noakes, P.S.
65592851-b158-4bb7-abc1-c453e098f5e2
van der Maarel, M.A.
9213e684-77aa-4626-9c7f-de41a46f35e4
Kremmyda, L.S.
19781569-bda8-478a-842e-d0e97cce6298
Vlachava, M.
e24c6fcd-7f45-4059-bd6b-3cb7398eef58
Diaper, N.D.
4fb72117-ec04-48e9-b375-3eb5cb4997f5
Miles, E.A.
20332899-ecdb-4214-95bc-922dde36d416
Eussen, S.R.
5d278da5-7631-4d57-a289-9f72abd85cda
Garssen, J.
28f33740-df6e-4764-a617-47a1edd03ec8
Willemsen, L.E.
3c85647c-2990-48f3-afe5-393816bb1448
Wilson, S.J.
21c6875d-6870-441b-ae7a-603562a646b8
Godfrey, K.M.
0931701e-fe2c-44b5-8f0d-ec5c7477a6fd
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
19 October 2011
van den Elsen, L.W.
557593d4-1e12-4b8b-ad37-f7188d202d01
Noakes, P.S.
65592851-b158-4bb7-abc1-c453e098f5e2
van der Maarel, M.A.
9213e684-77aa-4626-9c7f-de41a46f35e4
Kremmyda, L.S.
19781569-bda8-478a-842e-d0e97cce6298
Vlachava, M.
e24c6fcd-7f45-4059-bd6b-3cb7398eef58
Diaper, N.D.
4fb72117-ec04-48e9-b375-3eb5cb4997f5
Miles, E.A.
20332899-ecdb-4214-95bc-922dde36d416
Eussen, S.R.
5d278da5-7631-4d57-a289-9f72abd85cda
Garssen, J.
28f33740-df6e-4764-a617-47a1edd03ec8
Willemsen, L.E.
3c85647c-2990-48f3-afe5-393816bb1448
Wilson, S.J.
21c6875d-6870-441b-ae7a-603562a646b8
Godfrey, K.M.
0931701e-fe2c-44b5-8f0d-ec5c7477a6fd
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
van den Elsen, L.W., Noakes, P.S., van der Maarel, M.A., Kremmyda, L.S., Vlachava, M., Diaper, N.D., Miles, E.A., Eussen, S.R., Garssen, J., Willemsen, L.E., Wilson, S.J., Godfrey, K.M. and Calder, P.C.
(2011)
Salmon consumption by pregnant women reduces ex vivo umbilical cord endothelial cell activation.
American Journal of Clinical Nutrition, 94 (6), .
(doi:10.3945/ajcn.111.016592).
(PMID:22011457)
Abstract
Background: In vitro exposure of endothelial cells (ECs) to n−3 (omega-3) long-chain PUFAs (LCPUFAs) reduces cell adhesion molecule (CAM) expression. However, to our knowledge, no previous human studies have examined the influence of an altered diet on CAM expression.
Objective: We assessed whether salmon (rich in n−3 LCPUFAs) consumption twice a week during pregnancy affected offspring umbilical vein EC CAM expression.
Design: Women were randomly assigned to maintain their habitual diets or to consume 2 portions of salmon per week during pregnancy months 4–9. ECs were isolated from umbilical cord veins collected at birth and cultured. The cell surface expression of intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) was assessed by flow cytometry after the culture of ECs in the presence and absence of bacterial LPS for 24 h. Cytokine and growth factor concentrations in culture supernatant fluid were measured by using a multiplex assay.
Results: LPS increased the expression of VCAM-1 and the production of several cytokines and growth factors. The level of ICAM-1 expression per cell [ie, the median fluorescence intensity (MFI)] was increased by LPS stimulation in the control group (16.9 ± 2.4 compared with 135.3 ± 20.2; P < 0.001) and to a lesser extent in the salmon group (14.1 ± 3.8 compared with 65.8 ± 22.4; P = 0.037). The ICAM-1 MFI in the salmon group after LPS stimulation was lower than in the control group (P = 0.006).
Conclusion: Increased dietary salmon intake in pregnancy dampens offspring EC activation, which implicates a role for n−3 LCPUFAs in the suppression of inflammatory processes in humans. This trial was registered at clinicaltrials.gov as NCT00801502.
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Published date: 19 October 2011
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Faculty of Health Sciences
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Local EPrints ID: 209641
URI: http://eprints.soton.ac.uk/id/eprint/209641
ISSN: 0002-9165
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Date deposited: 31 Jan 2012 16:51
Last modified: 15 Mar 2024 02:50
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Author:
L.W. van den Elsen
Author:
P.S. Noakes
Author:
M.A. van der Maarel
Author:
L.S. Kremmyda
Author:
M. Vlachava
Author:
N.D. Diaper
Author:
S.R. Eussen
Author:
J. Garssen
Author:
L.E. Willemsen
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