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Salmon consumption by pregnant women reduces ex vivo umbilical cord endothelial cell activation

Record type: Article

Background: In vitro exposure of endothelial cells (ECs) to n?3 (omega-3) long-chain PUFAs (LCPUFAs) reduces cell adhesion molecule (CAM) expression. However, to our knowledge, no previous human studies have examined the influence of an altered diet on CAM expression.

Objective: We assessed whether salmon (rich in n?3 LCPUFAs) consumption twice a week during pregnancy affected offspring umbilical vein EC CAM expression.

Design: Women were randomly assigned to maintain their habitual diets or to consume 2 portions of salmon per week during pregnancy months 4–9. ECs were isolated from umbilical cord veins collected at birth and cultured. The cell surface expression of intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) was assessed by flow cytometry after the culture of ECs in the presence and absence of bacterial LPS for 24 h. Cytokine and growth factor concentrations in culture supernatant fluid were measured by using a multiplex assay.

Results: LPS increased the expression of VCAM-1 and the production of several cytokines and growth factors. The level of ICAM-1 expression per cell [ie, the median fluorescence intensity (MFI)] was increased by LPS stimulation in the control group (16.9 ± 2.4 compared with 135.3 ± 20.2; P < 0.001) and to a lesser extent in the salmon group (14.1 ± 3.8 compared with 65.8 ± 22.4; P = 0.037). The ICAM-1 MFI in the salmon group after LPS stimulation was lower than in the control group (P = 0.006).

Conclusion: Increased dietary salmon intake in pregnancy dampens offspring EC activation, which implicates a role for n?3 LCPUFAs in the suppression of inflammatory processes in humans.

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Citation

van den Elsen, L.W., Noakes, P.S., van der Maarel, M.A., Kremmyda, L.S., Vlachava, M., Diaper, N.D., Miles, E.A., Eussen, S.R., Garssen, J., Willemsen, L.E., Wilson, S.J., Godfrey, K.M. and Calder, P.C. (2011) Salmon consumption by pregnant women reduces ex vivo umbilical cord endothelial cell activation American Journal of Clinical Nutrition, 94, (6), pp. 1418-1425. (doi:10.3945/?ajcn.111.016592). (PMID:22011457).

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Published date: December 2011
Organisations: Faculty of Health Sciences

Identifiers

Local EPrints ID: 209641
URI: http://eprints.soton.ac.uk/id/eprint/209641
ISSN: 0002-9165
PURE UUID: 10e37c9e-2841-4dc2-854d-f6133d2155ab
ORCID for E.A. Miles: ORCID iD orcid.org/0000-0002-8643-0655
ORCID for K.M. Godfrey: ORCID iD orcid.org/0000-0002-4643-0618

Catalogue record

Date deposited: 31 Jan 2012 16:51
Last modified: 18 Jul 2017 10:46

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Contributors

Author: L.W. van den Elsen
Author: P.S. Noakes
Author: M.A. van der Maarel
Author: L.S. Kremmyda
Author: M. Vlachava
Author: N.D. Diaper
Author: E.A. Miles ORCID iD
Author: S.R. Eussen
Author: J. Garssen
Author: L.E. Willemsen
Author: S.J. Wilson
Author: K.M. Godfrey ORCID iD
Author: P.C. Calder

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