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Evidence for plasticity and structural mimicry at the immunoglobulin light chain-protein L interface

Graille, Marc, Harrison, Steven, Crump, Matthew P., Findlow, Stuart C., Housden, Nicholas G., Muller, Bruno H., Battail-Poirot, Nicole, Sibai, Geneviève, Sutton, Brian J., Taussig, Michael J., Jolivet-Reynaud, Colette, Gore, Michael G. and Stura, Enrico A. (2002) Evidence for plasticity and structural mimicry at the immunoglobulin light chain-protein L interface The Journal of Biological Chemistry, 277, (49), pp. 47500-47506. (doi:10.1074/jbc.M206105200).

Record type: Article

Abstract

The multidomain bacterial surface protein L (PpL) is a virulence factor expressed by only 10% of Peptostreptococcus magnus strains, and its expression is correlated with bacterial vaginosis. The molecular basis for its ability to recognize 60% of mammalian immunoglobulin light chain variable regions (V-L) has been described recently by x-ray crystallography, which suggested the presence of two V-L binding sites on each protein L domain (Graille, M., Stura, E. A., Housden, N.G., Beekingham, J.A., Bottomley, S. P., Beale, D., Taussig, M.J., Sutton, B.J., Gore, M. G., and Charbonnier, J. (2001) Structure, 679-687). Here, we report the crystal structure at 2.1 Angstrom resolution of a protein L mutant complexed to an Fab' fragment with only 50% of the VL residues interacting with PpL site 1 conserved. Comparison of the site 1 interface from both structures shows how protein L is able to accommodate these sequence differences and therefore bind to a large repertoire of Ig. The x-ray structure and NMR results confirm the existence of two V-L binding sites on a single protein L domain. These sites exhibit a remarkable structural mimicry of growth factors binding to their receptors. This could explain the protein L superantigenic activity on human B lymphocytes.

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Submitted date: 19 June 2002
Published date: 8 September 2002
Keywords: human growth hormone, X-ray diffraction, peptosreptoococcus, crystal-structure, binding domain, straphylococcus-aureus, extracellular domain, FAB fragment, EPO receptor, human-IGG

Identifiers

Local EPrints ID: 24026
URI: http://eprints.soton.ac.uk/id/eprint/24026
ISSN: 0021-9258
PURE UUID: ed12316c-cbcb-42a9-b8db-837fb51a9806

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Date deposited: 17 Mar 2006
Last modified: 17 Jul 2017 16:15

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Contributors

Author: Marc Graille
Author: Steven Harrison
Author: Matthew P. Crump
Author: Stuart C. Findlow
Author: Nicholas G. Housden
Author: Bruno H. Muller
Author: Nicole Battail-Poirot
Author: Geneviève Sibai
Author: Brian J. Sutton
Author: Michael J. Taussig
Author: Colette Jolivet-Reynaud
Author: Michael G. Gore
Author: Enrico A. Stura

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