In vitro inhibition of angiogenesis by prostasomes
In vitro inhibition of angiogenesis by prostasomes
Prostasomes are biologically active organelles that are secreted by human prostate epithelial cells, and it is believed that they have a role in prostatic disease. We studied the effect of prostasomes on the human umbilical vein endothelial cell (HUVEC)/Matrigel model of angiogenesis, and the association of labelled prostasomes with HUVECs. The growth inhibitory effect of prostasomes on HUVECs was assayed by spectrophotometric measurement of residual biomass. Preparations of HUVECs on a Matrigel base were exposed to prostasomes, and the development of capillary-like networks was quantified. Prostasomes were labelled with PKH-26, and cultured with HUVECs. Prostasomes were not shown to have a significant effect on HUVEC survival. Angiogenesis assays showed inhibition. The PKH-26-labelled particles were shown to have adhered to the HUVECs. This study adds the inhibition of an in vitro correlate of angiogenesis to the known actions of prostasomes.
prostasomes, angiogenesis, in vitro, HUVEC, PKH26
174-178
Delves, G.H.
6148e70a-8575-40e5-a875-2eda6577391c
Stewart, A.B.
b0ebb7e8-6846-4155-9203-28d13bdbf7be
Lwaleed, B.A.
e7c59131-82ad-4a14-a227-7370e91e3f21
Cooper, A.J.
8a21c297-eda3-4479-8e81-1de258c8e2a1
June 2005
Delves, G.H.
6148e70a-8575-40e5-a875-2eda6577391c
Stewart, A.B.
b0ebb7e8-6846-4155-9203-28d13bdbf7be
Lwaleed, B.A.
e7c59131-82ad-4a14-a227-7370e91e3f21
Cooper, A.J.
8a21c297-eda3-4479-8e81-1de258c8e2a1
Delves, G.H., Stewart, A.B., Lwaleed, B.A. and Cooper, A.J.
(2005)
In vitro inhibition of angiogenesis by prostasomes.
Prostate Cancer and Prostatic Diseases, 8, .
(doi:10.1038/sj.pcan.4500790).
Abstract
Prostasomes are biologically active organelles that are secreted by human prostate epithelial cells, and it is believed that they have a role in prostatic disease. We studied the effect of prostasomes on the human umbilical vein endothelial cell (HUVEC)/Matrigel model of angiogenesis, and the association of labelled prostasomes with HUVECs. The growth inhibitory effect of prostasomes on HUVECs was assayed by spectrophotometric measurement of residual biomass. Preparations of HUVECs on a Matrigel base were exposed to prostasomes, and the development of capillary-like networks was quantified. Prostasomes were labelled with PKH-26, and cultured with HUVECs. Prostasomes were not shown to have a significant effect on HUVEC survival. Angiogenesis assays showed inhibition. The PKH-26-labelled particles were shown to have adhered to the HUVECs. This study adds the inhibition of an in vitro correlate of angiogenesis to the known actions of prostasomes.
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Published date: June 2005
Keywords:
prostasomes, angiogenesis, in vitro, HUVEC, PKH26
Identifiers
Local EPrints ID: 24045
URI: http://eprints.soton.ac.uk/id/eprint/24045
ISSN: 1365-7852
PURE UUID: f760666f-2f71-4f03-b9e4-4e7cd3aab9f5
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Date deposited: 20 Mar 2006
Last modified: 06 Aug 2024 01:39
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Author:
G.H. Delves
Author:
A.B. Stewart
Author:
A.J. Cooper
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