The University of Southampton
University of Southampton Institutional Repository

Kainic acid induces rapid cell death followed by transiently reduced cell proliferation in the immature granule cell layer of rat organotypic hippocampal slice cultures

Kainic acid induces rapid cell death followed by transiently reduced cell proliferation in the immature granule cell layer of rat organotypic hippocampal slice cultures
Kainic acid induces rapid cell death followed by transiently reduced cell proliferation in the immature granule cell layer of rat organotypic hippocampal slice cultures
Brain injury due to seizures results in transiently increased cell proliferation and neurogenesis in the subgranular zone of the adult dentate gyrus. In contrast, the immature postnatal brain appears to be more resistant to cell death after seizure-induced brain injury and paradoxically reacts to seizures by reducing SGZ proliferation. Organotypic hippocampal slice cultures are a useful paradigm for modelling the early postnatal hippocampus. We have investigated the temporal relationship between cell death and cell proliferation after kainate in the granule cell layer of rat organotypic hippocampal slice cultures equivalent to post natal day 11 animals. We found stable numbers and densities of mature thionine stained cells in the granule cell layer over 72 h in control cultures grown in defined medium. We also found a slowly declining cell proliferation rate over the same time period under control conditions. We report evidence of early cell death in the granule cell layer after just 2 h exposure to 5 AM kainate, followed by a significant decrease in cell proliferation in the granule cell layer at 24 h. In contrast to control conditions, cell proliferation rose significantly in the kainate exposed cultures by 72 h back to levels seen at 2 h. There were no significant changes in cell labelling with antibody to activated caspase-3 between kainate treated and control cultures at any time point examined. Our results suggest that kainate-induced injury in the early postnatal hippocampus damages precursor cells contributing to a reduction in granule layer cell proliferation.
kainic acid, cell proliferation, dentate gyrus, organotypic hippocampal slice culture, BrdU, immunohistochemistry
0006-8993
111-119
Sadgrove, M.P.
5bab0ab8-3394-4b40-8030-7478cf32d7dc
Chad, J.E.
d220e55e-3c13-4d1d-ae9a-1cfae8ccfbe1
Gray, W.P.
e0ba5a65-bd74-4a4f-b640-5abf64fbb377
Sadgrove, M.P.
5bab0ab8-3394-4b40-8030-7478cf32d7dc
Chad, J.E.
d220e55e-3c13-4d1d-ae9a-1cfae8ccfbe1
Gray, W.P.
e0ba5a65-bd74-4a4f-b640-5abf64fbb377

Sadgrove, M.P., Chad, J.E. and Gray, W.P. (2005) Kainic acid induces rapid cell death followed by transiently reduced cell proliferation in the immature granule cell layer of rat organotypic hippocampal slice cultures. Brain Research, 1035 (2), 111-119. (doi:10.1016/j.brainres.2004.11.059).

Record type: Article

Abstract

Brain injury due to seizures results in transiently increased cell proliferation and neurogenesis in the subgranular zone of the adult dentate gyrus. In contrast, the immature postnatal brain appears to be more resistant to cell death after seizure-induced brain injury and paradoxically reacts to seizures by reducing SGZ proliferation. Organotypic hippocampal slice cultures are a useful paradigm for modelling the early postnatal hippocampus. We have investigated the temporal relationship between cell death and cell proliferation after kainate in the granule cell layer of rat organotypic hippocampal slice cultures equivalent to post natal day 11 animals. We found stable numbers and densities of mature thionine stained cells in the granule cell layer over 72 h in control cultures grown in defined medium. We also found a slowly declining cell proliferation rate over the same time period under control conditions. We report evidence of early cell death in the granule cell layer after just 2 h exposure to 5 AM kainate, followed by a significant decrease in cell proliferation in the granule cell layer at 24 h. In contrast to control conditions, cell proliferation rose significantly in the kainate exposed cultures by 72 h back to levels seen at 2 h. There were no significant changes in cell labelling with antibody to activated caspase-3 between kainate treated and control cultures at any time point examined. Our results suggest that kainate-induced injury in the early postnatal hippocampus damages precursor cells contributing to a reduction in granule layer cell proliferation.

Full text not available from this repository.

More information

Published date: 2005
Keywords: kainic acid, cell proliferation, dentate gyrus, organotypic hippocampal slice culture, BrdU, immunohistochemistry

Identifiers

Local EPrints ID: 24084
URI: https://eprints.soton.ac.uk/id/eprint/24084
ISSN: 0006-8993
PURE UUID: 8cfbd9fe-da53-47bc-9d98-2aeafbe77036
ORCID for J.E. Chad: ORCID iD orcid.org/0000-0001-6442-4281

Catalogue record

Date deposited: 22 Mar 2006
Last modified: 06 Jun 2018 13:17

Export record

Altmetrics

Contributors

Author: M.P. Sadgrove
Author: J.E. Chad ORCID iD
Author: W.P. Gray

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×