The University of Southampton
University of Southampton Institutional Repository

Cloning of a crustin-like, single whey-acidic-domain, antibacterial peptide from the haemocytes of the European lobster, Homarus gammarus, and its response to infection with bacteria.

Record type: Article

Degenerate PCR was used to isolate a 221-base pair nucleotide sequence of a new crustin-like antibacterial peptide from the haemocytes of the European lobster, Homarus gammarus. Rapid amplification of cDNA ends was used to extend the sequence to determine the complete open reading frame and untranslated regions. The inferred amino acid sequence of this peptide was found to be similar to crustin-like peptides isolated for several species of shrimp as well as the shore crab, Carcinus maenas. The sequence also contains a single-whey-acidic protein (WAP) domain, similar to novel antibacterial single-whey-acidic domain (SWD) peptides that have been recently described in the tiger shrimp, Penaeus monodon, and the Pacific white shrimp, Litopenaeus vannamei. Real-time PCR was used to analyse the expression of the gene coding for this peptide. The gene is up regulated after inoculation with the Gram-positive lobster pathogen Aerococcus viridans var. homari but down regulated after inoculation with the Gram-negative bacteria Listonella anguillarum. Phylogenetic analysis of this new peptide shows that it is most related to other antimicrobial crustin peptides and that the crustins are only distantly related to the antibacterial SWD peptides recently described.

PDF Hauton_et_al_Mol_Immunol_2006.pdf - Version of Record
Restricted to Registered users only
Download (332kB)

Citation

Hauton, C., Brockton, V. and Smith, V.J. (2005) Cloning of a crustin-like, single whey-acidic-domain, antibacterial peptide from the haemocytes of the European lobster, Homarus gammarus, and its response to infection with bacteria. Molecular Immunology, 43, (9), pp. 1490-1496. (doi:10.1016/j.molimm.2005.07.029).

More information

Published date: 6 September 2005
Additional Information: Short communication

Identifiers

Local EPrints ID: 24111
URI: http://eprints.soton.ac.uk/id/eprint/24111
ISSN: 0161-5890
PURE UUID: b4fd26af-7d01-4a61-8538-4de6e182b25e
ORCID for C. Hauton: ORCID iD orcid.org/0000-0002-2313-4226

Catalogue record

Date deposited: 22 Mar 2006
Last modified: 17 Jul 2017 16:14

Export record

Altmetrics

Contributors

Author: C. Hauton ORCID iD
Author: V. Brockton
Author: V.J. Smith

University divisions


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×