Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster (Homarus gammarus) granular haemocytes.
Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster (Homarus gammarus) granular haemocytes.
This paper presents data from an investigation of the mode of action of five different crustacean immunostimulants presented to European lobster (Homarus gammarus) granulocytes cultured in vitro. The experiments were designed to test whether or not the immunostimulants could cause the short-term upregulation of genes coding for immune proteins without causing the cells to degranulate. Quantitative measurements of mRNA transcript abundance were made using real-time PCR and it was first necessary to isolate the complete gene sequences coding for the proteins prophenoloxidase (proPO), ?-1,3-glucan binding protein (?GBP) and ?-actin ( ?-act) in the lobster. These sequences were used to design TaqMan™ primer and fluorescent probe sets. The presented data indicated that the majority of the tested immunostimulants did not induce the up-regulation of immune-related gene expression in the granulocytes in isolation. Alternative modes of action, including the in vivo up-regulation of gene expression in haemopoetic tissues, are discussed.
33-42
Hauton, C
7706f6ba-4497-42b2-8c6d-00df81676331
Hammond, J.A.
0b97a6b0-b826-4928-a98c-02621a9fe59d
Smith, V.J.
7dc3f0fa-e744-4b55-b852-7c8af4abdaa3
2005
Hauton, C
7706f6ba-4497-42b2-8c6d-00df81676331
Hammond, J.A.
0b97a6b0-b826-4928-a98c-02621a9fe59d
Smith, V.J.
7dc3f0fa-e744-4b55-b852-7c8af4abdaa3
Hauton, C, Hammond, J.A. and Smith, V.J.
(2005)
Real-time PCR quantification of the in vitro effects of crustacean immunostimulants on gene expression in lobster (Homarus gammarus) granular haemocytes.
Developmental and Comparative Immunology, 29 (1), .
(doi:10.1016/j.dci.2004.05.006).
Abstract
This paper presents data from an investigation of the mode of action of five different crustacean immunostimulants presented to European lobster (Homarus gammarus) granulocytes cultured in vitro. The experiments were designed to test whether or not the immunostimulants could cause the short-term upregulation of genes coding for immune proteins without causing the cells to degranulate. Quantitative measurements of mRNA transcript abundance were made using real-time PCR and it was first necessary to isolate the complete gene sequences coding for the proteins prophenoloxidase (proPO), ?-1,3-glucan binding protein (?GBP) and ?-actin ( ?-act) in the lobster. These sequences were used to design TaqMan™ primer and fluorescent probe sets. The presented data indicated that the majority of the tested immunostimulants did not induce the up-regulation of immune-related gene expression in the granulocytes in isolation. Alternative modes of action, including the in vivo up-regulation of gene expression in haemopoetic tissues, are discussed.
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Submitted date: 2 July 2004
Published date: 2005
Organisations:
Ocean and Earth Science
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Local EPrints ID: 24112
URI: http://eprints.soton.ac.uk/id/eprint/24112
ISSN: 0145-305X
PURE UUID: 08b9021d-c428-4029-ba7a-cd9e408c8a98
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Date deposited: 22 Mar 2006
Last modified: 16 Mar 2024 02:52
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Author:
J.A. Hammond
Author:
V.J. Smith
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