In vitro cytotoxicity of crustacean immunostimulants for lobster (Homarus gammarus) granulocytes demonstrated using the neutral red uptake assay
In vitro cytotoxicity of crustacean immunostimulants for lobster (Homarus gammarus) granulocytes demonstrated using the neutral red uptake assay
The neutral red uptake (NRU) cell viability assay was adapted for use with lobster Homarus gammarus (Linnaeus, 1758) granulocytes cultured in vitro. The assay was more sensitive than the conventional trypan blue exclusion assay and facilitated a higher sample throughput than subjective microscope-based assessments of cell viability. The NRU assay was demonstrated to have a linear response from 470 to at least 126 000 cells cm-2. It was used to investigate the acute cytotoxicity of 3 commercial and 2 candidate crustacean aquaculture immunostimulants on lobster granulocytes. All 5 stimulants had a cytotoxic action on the granulocytes and the toxic dose for some of these stimulants was found to be below their commercially prescribed dose. The long term energetic cost of the use of these stimulants and the concomitant potential for a reduction in growth rate of cultured decapod crustaceans, which is fundamental to the success of commercial aquaculture, is identified and discussed.
65-73
Hauton, C
7706f6ba-4497-42b2-8c6d-00df81676331
Smith, V.J.
7dc3f0fa-e744-4b55-b852-7c8af4abdaa3
Hauton, C
7706f6ba-4497-42b2-8c6d-00df81676331
Smith, V.J.
7dc3f0fa-e744-4b55-b852-7c8af4abdaa3
Hauton, C and Smith, V.J.
(2003)
In vitro cytotoxicity of crustacean immunostimulants for lobster (Homarus gammarus) granulocytes demonstrated using the neutral red uptake assay.
Fish and Shellfish Immunology, 17 (1), .
(doi:10.1016/j.fsi.2003.12.003).
(Submitted)
Abstract
The neutral red uptake (NRU) cell viability assay was adapted for use with lobster Homarus gammarus (Linnaeus, 1758) granulocytes cultured in vitro. The assay was more sensitive than the conventional trypan blue exclusion assay and facilitated a higher sample throughput than subjective microscope-based assessments of cell viability. The NRU assay was demonstrated to have a linear response from 470 to at least 126 000 cells cm-2. It was used to investigate the acute cytotoxicity of 3 commercial and 2 candidate crustacean aquaculture immunostimulants on lobster granulocytes. All 5 stimulants had a cytotoxic action on the granulocytes and the toxic dose for some of these stimulants was found to be below their commercially prescribed dose. The long term energetic cost of the use of these stimulants and the concomitant potential for a reduction in growth rate of cultured decapod crustaceans, which is fundamental to the success of commercial aquaculture, is identified and discussed.
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Submitted date: 12 December 2003
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Local EPrints ID: 24113
URI: http://eprints.soton.ac.uk/id/eprint/24113
PURE UUID: 2f36ace4-2959-4532-9e9c-f52e71b79ce7
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Date deposited: 22 Mar 2006
Last modified: 16 Mar 2024 02:52
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V.J. Smith
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