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In vitro cytotoxicity of crustacean immunostimulants for lobster (Homarus gammarus) granulocytes demonstrated using the neutral red uptake assay

Record type: Article

The neutral red uptake (NRU) cell viability assay was adapted for use with lobster Homarus gammarus (Linnaeus, 1758) granulocytes cultured in vitro. The assay was more sensitive than the conventional trypan blue exclusion assay and facilitated a higher sample throughput than subjective microscope-based assessments of cell viability. The NRU assay was demonstrated to have a linear response from 470 to at least 126 000 cells cm-2. It was used to investigate the acute cytotoxicity of 3 commercial and 2 candidate crustacean aquaculture immunostimulants on lobster granulocytes. All 5 stimulants had a cytotoxic action on the granulocytes and the toxic dose for some of these stimulants was found to be below their commercially prescribed dose. The long term energetic cost of the use of these stimulants and the concomitant potential for a reduction in growth rate of cultured decapod crustaceans, which is fundamental to the success of commercial aquaculture, is identified and discussed.

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Citation

Hauton, C and Smith, V.J. (2003) In vitro cytotoxicity of crustacean immunostimulants for lobster (Homarus gammarus) granulocytes demonstrated using the neutral red uptake assay Fish and Shellfish Immunology, 17, (1), pp. 65-73. (doi:10.1016/j.fsi.2003.12.003).

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Submitted date: 12 December 2003

Identifiers

Local EPrints ID: 24113
URI: http://eprints.soton.ac.uk/id/eprint/24113
PURE UUID: 2f36ace4-2959-4532-9e9c-f52e71b79ce7
ORCID for C Hauton: ORCID iD orcid.org/0000-0002-2313-4226

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Date deposited: 22 Mar 2006
Last modified: 17 Jul 2017 16:14

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Contributors

Author: C Hauton ORCID iD
Author: V.J. Smith

University divisions


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