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Nitric oxide modulates presynaptic afferent depolarization of mechanosensory neurons

Nitric oxide modulates presynaptic afferent depolarization of mechanosensory neurons
Nitric oxide modulates presynaptic afferent depolarization of mechanosensory neurons
In crayfish, movement of the tailfan causes stimulation of exteroceptive sensory hairs located on its surface. Movement is monitored by a proprioceptor,the protopodite-endopodite chordotonal organ within the tailfan. Proprioceptive afferents provide indirect presynaptic inhibitory inputs to sensory hair afferents in the form of primary afferent depolarizations (PADs). Bath application of nitric oxide (NO) substrates, donors and scavengers, and nitric oxide synthase (NOS) inhibitors had no effect on the responses of proprioceptive afferents during imposed movements of the chordotonal organ. In contrast, the amplitude of PADs inexteroceptive hair afferents was dependent on NO levels. NO levels were altered by bath-application of the NO precursor L-arginine, the NO donor SNAP, the NOS inhibitor L-NAME, and the NO scavenger PTIO, while changes in PAD amplitude were measured. Application of L-arginine or SNAP resulted in consistent decreases in PAD amplitude, whereas L-NAME and PTIO induced increases in PAD amplitude. These results suggest that endogenous NO decreases inhibitory inputs to exteroceptive neurons, thus enhancing transmitter release at their output synapses.
neuromodulation, presynaptic inhibition, local circuit, reflex, crayfish
0022-3034
331-342
Schuppe, Hansjürgen
17f82c3c-51da-46ff-a457-80d4f991b4ef
Newland, Philip L.
7a018c0e-37ba-40f5-bbf6-49ab0f299dbb
Schuppe, Hansjürgen
17f82c3c-51da-46ff-a457-80d4f991b4ef
Newland, Philip L.
7a018c0e-37ba-40f5-bbf6-49ab0f299dbb

Schuppe, Hansjürgen and Newland, Philip L. (2004) Nitric oxide modulates presynaptic afferent depolarization of mechanosensory neurons. Journal of Neurobiology, 59 (3), 331-342. (doi:10.1002/neu.10333).

Record type: Article

Abstract

In crayfish, movement of the tailfan causes stimulation of exteroceptive sensory hairs located on its surface. Movement is monitored by a proprioceptor,the protopodite-endopodite chordotonal organ within the tailfan. Proprioceptive afferents provide indirect presynaptic inhibitory inputs to sensory hair afferents in the form of primary afferent depolarizations (PADs). Bath application of nitric oxide (NO) substrates, donors and scavengers, and nitric oxide synthase (NOS) inhibitors had no effect on the responses of proprioceptive afferents during imposed movements of the chordotonal organ. In contrast, the amplitude of PADs inexteroceptive hair afferents was dependent on NO levels. NO levels were altered by bath-application of the NO precursor L-arginine, the NO donor SNAP, the NOS inhibitor L-NAME, and the NO scavenger PTIO, while changes in PAD amplitude were measured. Application of L-arginine or SNAP resulted in consistent decreases in PAD amplitude, whereas L-NAME and PTIO induced increases in PAD amplitude. These results suggest that endogenous NO decreases inhibitory inputs to exteroceptive neurons, thus enhancing transmitter release at their output synapses.

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Published date: June 2004
Keywords: neuromodulation, presynaptic inhibition, local circuit, reflex, crayfish

Identifiers

Local EPrints ID: 24212
URI: http://eprints.soton.ac.uk/id/eprint/24212
ISSN: 0022-3034
PURE UUID: fcf03127-f8d0-49fb-9763-065522be26c3
ORCID for Philip L. Newland: ORCID iD orcid.org/0000-0003-4124-8507

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Date deposited: 28 Mar 2006
Last modified: 09 Jan 2022 02:56

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Author: Hansjürgen Schuppe

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