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Nitric oxide modulates local reflexes of the tailfan of the crayfish

Nitric oxide modulates local reflexes of the tailfan of the crayfish
Nitric oxide modulates local reflexes of the tailfan of the crayfish
Electrical stimulation of sensory neurons that innervate receptors on the tailfan of crayfish evokes a reflex response of motor neurons that produce movements of the blades of the tailfan, the uropods. We analyzed the modulatory effects of nitric oxide (NO) on the spike frequency of the reflex response. Bath application of L-arginine and SNAP, which elevate endogenous and exogenous NO levels, increased the frequency of the evoked response, whereas the application of LNAME and PTIO, which reduce NO levels, decreased the frequency of the response. To determine through what pathway and target NO exerted these effects we bath applied ODQ, an inhibitor of soluble guanylyl cyclase (sGC), which decreased the frequency of response, and 8-br-cGMP, which increased the spike frequency of response. To provide further evidence that NO acts via sGC, we elevated NO levels with L-arginine while simultaneously inhibiting sGC with ODQ. This application reduced the response to control levels, indicating that NO in the terminal ganglion of crayfish acts via sGC to modulate cGMP levels, which in turn regulate the responses of the uropod motor neurons.
cyclic GMP, modulation, motor neuron, reflex, local circuits
0022-3034
176-186
Araki, M.
fe931e60-78e0-4d55-a97a-33829b122bf4
Schuppe, H.
653d5319-bc95-4919-b401-8c56e51cc60e
Fujimoto, S.
3143a30d-6269-4eab-b75f-db299576dd41
Nagayama, T.
eaa8380e-589f-465e-ab43-a98881924846
Newland, P.L.
7a018c0e-37ba-40f5-bbf6-49ab0f299dbb
Araki, M.
fe931e60-78e0-4d55-a97a-33829b122bf4
Schuppe, H.
653d5319-bc95-4919-b401-8c56e51cc60e
Fujimoto, S.
3143a30d-6269-4eab-b75f-db299576dd41
Nagayama, T.
eaa8380e-589f-465e-ab43-a98881924846
Newland, P.L.
7a018c0e-37ba-40f5-bbf6-49ab0f299dbb

Araki, M., Schuppe, H., Fujimoto, S., Nagayama, T. and Newland, P.L. (2004) Nitric oxide modulates local reflexes of the tailfan of the crayfish. Journal of Neurobiology, 60 (2), 176-186. (doi:10.1002/neu.20007).

Record type: Article

Abstract

Electrical stimulation of sensory neurons that innervate receptors on the tailfan of crayfish evokes a reflex response of motor neurons that produce movements of the blades of the tailfan, the uropods. We analyzed the modulatory effects of nitric oxide (NO) on the spike frequency of the reflex response. Bath application of L-arginine and SNAP, which elevate endogenous and exogenous NO levels, increased the frequency of the evoked response, whereas the application of LNAME and PTIO, which reduce NO levels, decreased the frequency of the response. To determine through what pathway and target NO exerted these effects we bath applied ODQ, an inhibitor of soluble guanylyl cyclase (sGC), which decreased the frequency of response, and 8-br-cGMP, which increased the spike frequency of response. To provide further evidence that NO acts via sGC, we elevated NO levels with L-arginine while simultaneously inhibiting sGC with ODQ. This application reduced the response to control levels, indicating that NO in the terminal ganglion of crayfish acts via sGC to modulate cGMP levels, which in turn regulate the responses of the uropod motor neurons.

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Submitted date: 17 September 2003
Published date: August 2004
Keywords: cyclic GMP, modulation, motor neuron, reflex, local circuits
Organisations: Biological Sciences

Identifiers

Local EPrints ID: 24214
URI: http://eprints.soton.ac.uk/id/eprint/24214
ISSN: 0022-3034
PURE UUID: 6f95aef5-a4ea-4cb8-b2da-bfec8c444927
ORCID for P.L. Newland: ORCID iD orcid.org/0000-0003-4124-8507

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Date deposited: 28 Mar 2006
Last modified: 18 Jun 2021 01:35

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Contributors

Author: M. Araki
Author: H. Schuppe
Author: S. Fujimoto
Author: T. Nagayama
Author: P.L. Newland ORCID iD

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