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Proteomic detection of PhoPQ- and acid-mediated repression of Salmonella motility

Proteomic detection of PhoPQ- and acid-mediated repression of Salmonella motility
Proteomic detection of PhoPQ- and acid-mediated repression of Salmonella motility
Salmonella adaptation to low pH is a critical survival response and essential for virulence. Here, we show that another key virulence-associated process, flagellamediated cell motility, is co-regulated by low pH via the PhoPQ signal transduction system. Using a proteomic approach, we found that phase 1 and phase 2 flagellin were specifically down-regulated when acid-adapted (pH 5.0) Salmonella SL1344 cells were exposed to pH 3.0. Decreased flagellin expression and cell motility was dependent on activation of the PhoPQ pathway, which directly or indirectly negatively regulated transcription of the flagellin gene fliC. In contrast, the general stress sigma factor RpoS (S) positively regulated flagellar gene expression. Low external pH had no effect on the level of H-NS protein, a further regulator of flagellar gene expression. We suggest that flagellar repression at low pH conserves ATP for survival processes and helps to limit the influx of protons into the cytosol. These results highlight the power of proteomics to reveal unanticipated links between relatively well-characterised regulatory systems in bacteria.
salmonella, cell motility, acid tolerance
1615-9853
597-607
Adams, Phillip
09d93c06-1277-4720-ba46-8d8808531b99
Fowler, Richard
49599182-ebf9-4d19-b7c2-81bfc765f542
Kinsella, Niamh
e359ba03-c2a2-43db-af54-bb0289290b3d
Howell, Gillian
a7c9bc56-3393-4903-8621-fe8c215ff08b
Farris, Michele
c5aede6f-7041-4109-ba6d-b3ab42291671
Coote, Peter
1799e4bd-84d3-4d3f-970b-01be4d617d96
O'Connor, David
7c29ec66-081e-46f0-8be8-bac6a7dc8de8
Adams, Phillip
09d93c06-1277-4720-ba46-8d8808531b99
Fowler, Richard
49599182-ebf9-4d19-b7c2-81bfc765f542
Kinsella, Niamh
e359ba03-c2a2-43db-af54-bb0289290b3d
Howell, Gillian
a7c9bc56-3393-4903-8621-fe8c215ff08b
Farris, Michele
c5aede6f-7041-4109-ba6d-b3ab42291671
Coote, Peter
1799e4bd-84d3-4d3f-970b-01be4d617d96
O'Connor, David
7c29ec66-081e-46f0-8be8-bac6a7dc8de8

Adams, Phillip, Fowler, Richard, Kinsella, Niamh, Howell, Gillian, Farris, Michele, Coote, Peter and O'Connor, David (2001) Proteomic detection of PhoPQ- and acid-mediated repression of Salmonella motility. Proteomics, 1 (4), 597-607.

Record type: Article

Abstract

Salmonella adaptation to low pH is a critical survival response and essential for virulence. Here, we show that another key virulence-associated process, flagellamediated cell motility, is co-regulated by low pH via the PhoPQ signal transduction system. Using a proteomic approach, we found that phase 1 and phase 2 flagellin were specifically down-regulated when acid-adapted (pH 5.0) Salmonella SL1344 cells were exposed to pH 3.0. Decreased flagellin expression and cell motility was dependent on activation of the PhoPQ pathway, which directly or indirectly negatively regulated transcription of the flagellin gene fliC. In contrast, the general stress sigma factor RpoS (S) positively regulated flagellar gene expression. Low external pH had no effect on the level of H-NS protein, a further regulator of flagellar gene expression. We suggest that flagellar repression at low pH conserves ATP for survival processes and helps to limit the influx of protons into the cytosol. These results highlight the power of proteomics to reveal unanticipated links between relatively well-characterised regulatory systems in bacteria.

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Published date: April 2001
Keywords: salmonella, cell motility, acid tolerance

Identifiers

Local EPrints ID: 24221
URI: https://eprints.soton.ac.uk/id/eprint/24221
ISSN: 1615-9853
PURE UUID: 11cad643-fb2d-473c-9a1b-164c5eaaa05f

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Date deposited: 28 Mar 2006
Last modified: 06 Nov 2017 13:25

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