A dedicated translation factor controls the synthesis of the global regulator Fis

Owens, R., Pritchard, G., Skipp, P., Hodey, M., Connell, S.R., Nierhaus, K.H. and O'Connor, D. (2004) A dedicated translation factor controls the synthesis of the global regulator Fis The EMBO Journal, 23, pp. 3375-3385. (doi:10.1038/sj.emboj.7600343).


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BipA is a highly conserved protein with global regulatory properties in Escherichia coli. We show here that it functions as a translation factor that is required specifically for the expression of the transcriptional modulator Fis. BipA binds to ribosomes at a site that coincides with that of elongation factor G and has a GTPase activity that is sensitive to high GDP:GTP ratios and stimulated by 70S ribosomes programmed with mRNA and aminoacylated tRNAs. The growth rate-dependent induction of BipA allows the efficient expression of Fis, thereby modulating a range of downstream processes, including DNA metabolism and type III secretion. We propose a model in which BipA destabilizes unusually strong interactions between the 50 untranslated region of fis mRNA and the ribosome. Since BipA spans phylogenetic domains, transcript-selective translational control for the ‘fast-track’ expression of specific mRNAs may have wider significance.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1038/sj.emboj.7600343
ISSNs: 0261-4189 (print)
Related URLs:
Keywords: proteins, cellular metabolism Keywords: Fis, GTPase, regulation, ribosome, translation
ePrint ID: 24222
Date :
Date Event
5 August 2004Published
Date Deposited: 28 Mar 2006
Last Modified: 16 Apr 2017 22:41
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/24222

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