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A dedicated translation factor controls the synthesis of the global regulator Fis

Owens, Róisín M., Pritchard, Gareth, Skipp, Paul, Hodey, Michelle, Connell, Sean R., Nierhaus, Knud H. and Connor, C. David (2004) A dedicated translation factor controls the synthesis of the global regulator Fis The EMBO Journal, 23, (16), pp. 3375-3385. (doi:10.1038/sj.emboj.7600343).

Record type: Article

Abstract

BipA is a highly conserved protein with global regulatory properties in Escherichia coli. We show here that it functions as a translation factor that is required specifically for the expression of the transcriptional modulator Fis. BipA binds to ribosomes at a site that coincides with that of elongation factor G and has a GTPase activity that is sensitive to high GDP:GTP ratios and stimulated by 70S ribosomes programmed with mRNA and aminoacylated tRNAs. The growth rate-dependent induction of BipA allows the efficient expression of Fis, thereby modulating a range of downstream processes, including DNA metabolism and type III secretion. We propose a model in which BipA destabilizes unusually strong interactions between the 50 untranslated region of fis mRNA and the ribosome. Since BipA spans phylogenetic domains, transcript-selective translational control for the ‘fast-track’ expression of specific mRNAs may have wider significance.

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Published date: 5 August 2004
Keywords: proteins, cellular metabolism Keywords: Fis, GTPase, regulation, ribosome, translation
Organisations: Biological Sciences

Identifiers

Local EPrints ID: 24222
URI: http://eprints.soton.ac.uk/id/eprint/24222
ISSN: 0261-4189
PURE UUID: 45f50d0f-d3b0-41a5-85f1-a1473017c78f
ORCID for Paul Skipp: ORCID iD orcid.org/0000-0002-2995-2959

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Date deposited: 28 Mar 2006
Last modified: 17 Jul 2017 16:14

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Contributors

Author: Róisín M. Owens
Author: Gareth Pritchard
Author: Paul Skipp ORCID iD
Author: Michelle Hodey
Author: Sean R. Connell
Author: Knud H. Nierhaus
Author: C. David Connor

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