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Retracted. A dedicated translation factor controls the synthesis of the global regulator Fis

Retracted. A dedicated translation factor controls the synthesis of the global regulator Fis
Retracted. A dedicated translation factor controls the synthesis of the global regulator Fis
Retraction to: The EMBO Journal (2004) 23, 3375–3385. doi:10.1038/sj.emboj.7600343
In the above report, we presented data indicating that BipA, a protein that shares sequence identity with translational elongation factors, is required for the expression of the transcriptional modulator Fis. As part of our ongoing studies into BipA, we discovered that we were unable to reproduce data presented in Figures 4 and 6C of the paper, which were generated by JGP when he was in CDOC's laboratory. Specifically, we have been unable to reproduce immunoblots indicating that BipA is required for the efficient expression of Fis protein and, using a reconstructed version of the plasmid pJGP1, find that there is no significant difference in the level of Fis translation in the presence or absence of BipA. While we are confident that the experiments concerning the interactions of BipA with 70S ribosomes are valid, we conclude that the results shown in Figures 4 and 6C are unsound and therefore wish to retract the paper.

Original Abstract:
BipA is a highly conserved protein with global regulatoryproperties in Escherichia coli. We show here that it functionsas a translation factor that is required specifically forthe expression of the transcriptional modulator Fis. BipAbinds to ribosomes at a site that coincides with that ofelongation factor G and has a GTPase activity that issensitive to high GDP:GTP ratios and stimulated by 70Sribosomes programmed with mRNA and aminoacylatedtRNAs. The growth rate-dependent induction of BipAallows the efficient expression of Fis, thereby modulatinga range of downstream processes, including DNA metabolismand type III secretion. We propose a model in whichBipA destabilizes unusually strong interactions betweenthe 50 untranslated region of fis mRNA and the ribosome.Since BipA spans phylogenetic domains, transcript-selectivetranslational control for the ‘fast-track’ expression ofspecific mRNAs may have wider significance.
proteins, cellular metabolismKeywords: Fis, GTPase, regulation, ribosome, translation
0261-4189
3375-3385
Owens, Róisín M.
158f1423-44d9-4869-8e8e-c5bc888a82c5
Pritchard, Gareth
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Skipp, Paul
1ba7dcf6-9fe7-4b5c-a9d0-e32ed7f42aa5
Hodey, Michelle
35bca552-6b69-4308-9142-7d6df7c0826d
Connell, Sean R.
18a63297-0ef1-4bb4-8344-a6dd6a5aca16
Nierhaus, Knud H.
dbe433aa-7ce9-4acd-af51-1b0c625aafd7
Connor, C. David
275a4599-af73-42f4-b91c-e75cf884eecc
Owens, Róisín M.
158f1423-44d9-4869-8e8e-c5bc888a82c5
Pritchard, Gareth
a75392c4-5283-4b54-bcd5-24d1e3bb9a95
Skipp, Paul
1ba7dcf6-9fe7-4b5c-a9d0-e32ed7f42aa5
Hodey, Michelle
35bca552-6b69-4308-9142-7d6df7c0826d
Connell, Sean R.
18a63297-0ef1-4bb4-8344-a6dd6a5aca16
Nierhaus, Knud H.
dbe433aa-7ce9-4acd-af51-1b0c625aafd7
Connor, C. David
275a4599-af73-42f4-b91c-e75cf884eecc

Owens, Róisín M., Pritchard, Gareth, Skipp, Paul, Hodey, Michelle, Connell, Sean R., Nierhaus, Knud H. and Connor, C. David (2004) Retracted. A dedicated translation factor controls the synthesis of the global regulator Fis. The EMBO Journal, 23 (16), 3375-3385. (doi:10.1038/sj.emboj.7600343).

Record type: Article

Abstract

Retraction to: The EMBO Journal (2004) 23, 3375–3385. doi:10.1038/sj.emboj.7600343
In the above report, we presented data indicating that BipA, a protein that shares sequence identity with translational elongation factors, is required for the expression of the transcriptional modulator Fis. As part of our ongoing studies into BipA, we discovered that we were unable to reproduce data presented in Figures 4 and 6C of the paper, which were generated by JGP when he was in CDOC's laboratory. Specifically, we have been unable to reproduce immunoblots indicating that BipA is required for the efficient expression of Fis protein and, using a reconstructed version of the plasmid pJGP1, find that there is no significant difference in the level of Fis translation in the presence or absence of BipA. While we are confident that the experiments concerning the interactions of BipA with 70S ribosomes are valid, we conclude that the results shown in Figures 4 and 6C are unsound and therefore wish to retract the paper.

Original Abstract:
BipA is a highly conserved protein with global regulatoryproperties in Escherichia coli. We show here that it functionsas a translation factor that is required specifically forthe expression of the transcriptional modulator Fis. BipAbinds to ribosomes at a site that coincides with that ofelongation factor G and has a GTPase activity that issensitive to high GDP:GTP ratios and stimulated by 70Sribosomes programmed with mRNA and aminoacylatedtRNAs. The growth rate-dependent induction of BipAallows the efficient expression of Fis, thereby modulatinga range of downstream processes, including DNA metabolismand type III secretion. We propose a model in whichBipA destabilizes unusually strong interactions betweenthe 50 untranslated region of fis mRNA and the ribosome.Since BipA spans phylogenetic domains, transcript-selectivetranslational control for the ‘fast-track’ expression ofspecific mRNAs may have wider significance.

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Published date: 5 August 2004
Keywords: proteins, cellular metabolismKeywords: Fis, GTPase, regulation, ribosome, translation
Organisations: Biological Sciences

Identifiers

Local EPrints ID: 24222
URI: http://eprints.soton.ac.uk/id/eprint/24222
ISSN: 0261-4189
PURE UUID: 45f50d0f-d3b0-41a5-85f1-a1473017c78f
ORCID for Paul Skipp: ORCID iD orcid.org/0000-0002-2995-2959

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Date deposited: 28 Mar 2006
Last modified: 19 Jul 2022 01:35

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Contributors

Author: Róisín M. Owens
Author: Gareth Pritchard
Author: Paul Skipp ORCID iD
Author: Michelle Hodey
Author: Sean R. Connell
Author: Knud H. Nierhaus
Author: C. David Connor

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