The University of Southampton
University of Southampton Institutional Repository

A dedicated translation factor controls the synthesis of the global regulator Fis

Owens, Róisín M., Pritchard, Gareth, Skipp, Paul, Hodey, Michelle, Connell, Sean R., Nierhaus, Knud H. and Connor, C. David (2004) A dedicated translation factor controls the synthesis of the global regulator Fis The EMBO Journal, 23, (16), pp. 3375-3385. (doi:10.1038/sj.emboj.7600343).

Record type: Article


BipA is a highly conserved protein with global regulatory properties in Escherichia coli. We show here that it functions as a translation factor that is required specifically for the expression of the transcriptional modulator Fis. BipA binds to ribosomes at a site that coincides with that of elongation factor G and has a GTPase activity that is sensitive to high GDP:GTP ratios and stimulated by 70S ribosomes programmed with mRNA and aminoacylated tRNAs. The growth rate-dependent induction of BipA allows the efficient expression of Fis, thereby modulating a range of downstream processes, including DNA metabolism and type III secretion. We propose a model in which BipA destabilizes unusually strong interactions between the 50 untranslated region of fis mRNA and the ribosome. Since BipA spans phylogenetic domains, transcript-selective translational control for the ‘fast-track’ expression of specific mRNAs may have wider significance.

PDF doc2.pdf - Other
Restricted to Registered users only
Download (259kB)

More information

Published date: 5 August 2004
Keywords: proteins, cellular metabolism Keywords: Fis, GTPase, regulation, ribosome, translation
Organisations: Biological Sciences


Local EPrints ID: 24222
ISSN: 0261-4189
PURE UUID: 45f50d0f-d3b0-41a5-85f1-a1473017c78f
ORCID for Paul Skipp: ORCID iD

Catalogue record

Date deposited: 28 Mar 2006
Last modified: 17 Jul 2017 16:14

Export record



Author: Róisín M. Owens
Author: Gareth Pritchard
Author: Paul Skipp ORCID iD
Author: Michelle Hodey
Author: Sean R. Connell
Author: Knud H. Nierhaus
Author: C. David Connor

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.