Specific PKC isoforms regulate blastocoel formation during mouse preimplantation developmentpreimplantation development


Eckert, Judith J., McCallum, Amanda, Mears, Andrew, Rumsby, Martin G., Cameron, Iain T. and Fleming, Tom P. (2004) Specific PKC isoforms regulate blastocoel formation during mouse preimplantation developmentpreimplantation development Developmental Biology, 274, (2), pp. 384-401. (doi:10.1016/j.ydbio.2004.07.027).

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Description/Abstract

During early mammalian development, blastocyst morphogenesis is achieved by epithelial differentiation of trophectoderm (TE) and its segregation from the inner cell mass (ICM). Two major interrelated features of TE differentiation required for blastocoel formation include intercellular junction biogenesis and a directed ion transport system, mediated by Na+/K+ ATPase. We have examined the relative contribution of intercellular signalling mediated by protein kinase C (PKC) and gap junctional communication in TE differentiation and blastocyst cavitation. The distribution pattern of four (y, u, L/E, ~) PKC isoforms and PKCA/PKD1 showed partial colocalisation with the tight junction marker ZO-1a+ in TE and all four PKCs (y, u, L/E, ~) showed distinct TE/ICM staining patterns (predominantly at the cell membrane within the TE and cytoplasmic within the ICM), indicating their potential contribution to TE differentiation and blastocyst morphogenesis. Specific inhibition of PKCy and ~ activity significantly delayed blastocyst formation. Although modulation of these PKC isoforms failed to influence the already established programme of epithelial junctional differentiation within the TE, Na+/K+ ATPase a1 subunit was internalised from membrane to cytoplasm. Inhibition of gap junctional communication, in contrast, had no influence on any of these processes. Our results demonstrate for the first time that distinct PKC isotypes contribute to the regulation of cavitation in preimplantation embryos via target proteins including Na+/K+ ATPase.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1016/j.ydbio.2004.07.027
ISSNs: 0012-1606 (print)
Related URLs:
Keywords: protein kinase C, mouse embryo, blastocyst, trophectoderm, inner cell mass, tight junction, ZO-1, Na+/K+ ATPase, cavitation
Subjects:
ePrint ID: 24227
Date :
Date Event
20 May 2004Submitted
15 October 2004Published
Date Deposited: 29 Mar 2006
Last Modified: 16 Apr 2017 22:41
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/24227

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