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Peripheral blood mononuclear cell fatty acid composition and inflammatory mediator production in adult Crohn's disease

Peripheral blood mononuclear cell fatty acid composition and inflammatory mediator production in adult Crohn's disease
Peripheral blood mononuclear cell fatty acid composition and inflammatory mediator production in adult Crohn's disease
Background & aims: Crohn's disease (CD) is associated with nutritional deficiencies, altered plasma concentrations of polyunsaturated fatty acids (PUFA) and an anti-inflammatory response to fish oil that contains n-3 PUFA. This suggests that, in CD, immune cells may have altered n-3 PUFA composition with functional consequences. The aim of this study is to investigate n-3 and n-6 PUFA composition and synthetic function of peripheral blood mononuclear cells (PBMC) in the basal state.
Methods: A case control study of 52 adult CD patients and healthy, age- and sex-matched controls. Composition of PBMC and plasma phospholipids were measured by gas chromatography and production of tumour necrosis factor-?, prostaglandin E2 (PGE2) and interferon-? (IFN-?) by PBMC were measured by ELISA.
Results: CD was associated with higher concentrations of eicosapentaenoic acid and other n-3 PUFA, and lower arachidonic acid (AA) (n-6 PUFA) in PBMC. This was not explained by differences in dietary fat intake. Lower rates of production of PGE2 and IFN-? by PBMC were noted in quiescent and active CD, respectively, compared to controls.
Conclusions: CD is associated with a greater availability, and not a deficiency, of n-3 PUFA in PBMC, but lower concentrations of AA, and lower rates of production of PGE2 and IFN-?, compared to healthy controls.
mononuclear cell, cytokines, polyunsaturated fatty acids, crohn's disease
0261-5614
647-655
Trebble, Timothy M.
48119242-d090-4778-afd9-58f451dbeecf
Arden, Nigel K.
23af958d-835c-4d79-be54-4bbe4c68077f
Wootton, Stephen A.
bf47ef35-0b33-4edb-a2b0-ceda5c475c0c
Mullee, Mark A.
fd3f91c3-5e95-4f56-8d73-260824eeb362
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Burdge, Graham C.
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
Fine, David R.
d902838f-1061-4228-bdab-6358a951cb15
Stroud, Mike A.
1665ae65-0898-4848-bf0d-baec8f2bb078
Trebble, Timothy M.
48119242-d090-4778-afd9-58f451dbeecf
Arden, Nigel K.
23af958d-835c-4d79-be54-4bbe4c68077f
Wootton, Stephen A.
bf47ef35-0b33-4edb-a2b0-ceda5c475c0c
Mullee, Mark A.
fd3f91c3-5e95-4f56-8d73-260824eeb362
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Burdge, Graham C.
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
Fine, David R.
d902838f-1061-4228-bdab-6358a951cb15
Stroud, Mike A.
1665ae65-0898-4848-bf0d-baec8f2bb078

Trebble, Timothy M., Arden, Nigel K., Wootton, Stephen A., Mullee, Mark A., Calder, Philip C., Burdge, Graham C., Fine, David R. and Stroud, Mike A. (2004) Peripheral blood mononuclear cell fatty acid composition and inflammatory mediator production in adult Crohn's disease. Clinical Nutrition, 23 (4), 647-655. (doi:10.1016/j.clnu.2003.10.017).

Record type: Article

Abstract

Background & aims: Crohn's disease (CD) is associated with nutritional deficiencies, altered plasma concentrations of polyunsaturated fatty acids (PUFA) and an anti-inflammatory response to fish oil that contains n-3 PUFA. This suggests that, in CD, immune cells may have altered n-3 PUFA composition with functional consequences. The aim of this study is to investigate n-3 and n-6 PUFA composition and synthetic function of peripheral blood mononuclear cells (PBMC) in the basal state.
Methods: A case control study of 52 adult CD patients and healthy, age- and sex-matched controls. Composition of PBMC and plasma phospholipids were measured by gas chromatography and production of tumour necrosis factor-?, prostaglandin E2 (PGE2) and interferon-? (IFN-?) by PBMC were measured by ELISA.
Results: CD was associated with higher concentrations of eicosapentaenoic acid and other n-3 PUFA, and lower arachidonic acid (AA) (n-6 PUFA) in PBMC. This was not explained by differences in dietary fat intake. Lower rates of production of PGE2 and IFN-? by PBMC were noted in quiescent and active CD, respectively, compared to controls.
Conclusions: CD is associated with a greater availability, and not a deficiency, of n-3 PUFA in PBMC, but lower concentrations of AA, and lower rates of production of PGE2 and IFN-?, compared to healthy controls.

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More information

Published date: August 2004
Keywords: mononuclear cell, cytokines, polyunsaturated fatty acids, crohn's disease

Identifiers

Local EPrints ID: 24530
URI: http://eprints.soton.ac.uk/id/eprint/24530
ISSN: 0261-5614
PURE UUID: f6b19527-f750-4bf4-99a1-00fff40e0eb2
ORCID for Philip C. Calder: ORCID iD orcid.org/0000-0002-6038-710X
ORCID for Graham C. Burdge: ORCID iD orcid.org/0000-0002-7665-2967

Catalogue record

Date deposited: 31 Mar 2006
Last modified: 08 Jan 2022 02:42

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Contributors

Author: Timothy M. Trebble
Author: Nigel K. Arden
Author: Mark A. Mullee
Author: David R. Fine
Author: Mike A. Stroud

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