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Eml5, a novel WD40 domain protein expressed in rat brain

Eml5, a novel WD40 domain protein expressed in rat brain
Eml5, a novel WD40 domain protein expressed in rat brain
We have isolated a novel transcript with homology to the major microtubule-associated protein in dividing sea urchin embryos, EMAP. The protein has a predicted MW of similar to 180 kDa and we have named it Eml5 (EMAP-like protein 5, GenBank accession no. AY445136). Eml5 contains 11 putative WD40 domains and 3 hydrophobic stretches of 43 aa, HELP domains, which have been suggested to be involved in microtubule binding. Eml5 appears to consist of two tandem repeats of the complete EMAP protein separated by a putative dimerization domain. Eml5 mRNA and protein is expressed at high levels in the hippocampus, cerebellum and olfactory bulb, as determined by in situ hybridization and immunocytochemistry. Eml5 transcripts can be detected in fore- and hindbrain structures from embryonic day 13 onwards. Because other EMAP-like proteins are involved in regulating microtubule dynamics, it is likely that Eml5 plays a role in the regulation of cytoskeletal rearrangements during neuronal development and in adult brain.
Abbreviations: EMAP, echinoderm microtubule-associated protein; EML, EMAP-like protein; MAP, microtubule-associated protein; HELP domain, hydrophobic EMAP-like protein domain; LTP, long-term potentiation; PSD, post-synaptic density.
neuronal expression, EMAP, MAPs, cytoskeleton, microtubule-destabilizing protein
0378-1119
127-137
O'Connor, V.
8021b06c-01a0-4925-9dde-a61c8fe278ca
Houtman, S.H.
959073c2-0c59-4c16-ab78-8247903466d5
De Zeeuw, C.I.
7741b2e6-f5f8-4a12-b2f4-4c398d9e5f24
Bliss, T.V.P
48693c5e-3c5d-4766-8aee-a0f86c1355be
French, P.J.
10c75469-53aa-449c-b8d6-9ef444b697b7
O'Connor, V.
8021b06c-01a0-4925-9dde-a61c8fe278ca
Houtman, S.H.
959073c2-0c59-4c16-ab78-8247903466d5
De Zeeuw, C.I.
7741b2e6-f5f8-4a12-b2f4-4c398d9e5f24
Bliss, T.V.P
48693c5e-3c5d-4766-8aee-a0f86c1355be
French, P.J.
10c75469-53aa-449c-b8d6-9ef444b697b7

O'Connor, V., Houtman, S.H., De Zeeuw, C.I., Bliss, T.V.P and French, P.J. (2004) Eml5, a novel WD40 domain protein expressed in rat brain. Gene, 336 (1), 127-137. (doi:10.1016/j.gene.2004.04.012).

Record type: Article

Abstract

We have isolated a novel transcript with homology to the major microtubule-associated protein in dividing sea urchin embryos, EMAP. The protein has a predicted MW of similar to 180 kDa and we have named it Eml5 (EMAP-like protein 5, GenBank accession no. AY445136). Eml5 contains 11 putative WD40 domains and 3 hydrophobic stretches of 43 aa, HELP domains, which have been suggested to be involved in microtubule binding. Eml5 appears to consist of two tandem repeats of the complete EMAP protein separated by a putative dimerization domain. Eml5 mRNA and protein is expressed at high levels in the hippocampus, cerebellum and olfactory bulb, as determined by in situ hybridization and immunocytochemistry. Eml5 transcripts can be detected in fore- and hindbrain structures from embryonic day 13 onwards. Because other EMAP-like proteins are involved in regulating microtubule dynamics, it is likely that Eml5 plays a role in the regulation of cytoskeletal rearrangements during neuronal development and in adult brain.
Abbreviations: EMAP, echinoderm microtubule-associated protein; EML, EMAP-like protein; MAP, microtubule-associated protein; HELP domain, hydrophobic EMAP-like protein domain; LTP, long-term potentiation; PSD, post-synaptic density.

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Published date: 7 July 2004
Keywords: neuronal expression, EMAP, MAPs, cytoskeleton, microtubule-destabilizing protein

Identifiers

Local EPrints ID: 24574
URI: http://eprints.soton.ac.uk/id/eprint/24574
ISSN: 0378-1119
PURE UUID: a833ef5b-195e-4f80-a43a-4d2baa4372d1
ORCID for V. O'Connor: ORCID iD orcid.org/0000-0003-3185-5709

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Date deposited: 31 Mar 2006
Last modified: 29 Mar 2022 01:36

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Contributors

Author: V. O'Connor ORCID iD
Author: S.H. Houtman
Author: C.I. De Zeeuw
Author: T.V.P Bliss
Author: P.J. French

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