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LTP but not seizure is associated with up-regulation of AKAP-150

LTP but not seizure is associated with up-regulation of AKAP-150
LTP but not seizure is associated with up-regulation of AKAP-150
We have used differential display to profile and compare the mRNAs expressed in the hippocampus of freely moving animals after the induction of long-term potentiation (LTP) at the perforant path–dentate gyrus synapse with control rats receiving low-frequency stimulation. We have combined this with in situ hybridization and have identified A-kinase anchoring protein of 150 kDa (AKAP-150) as a gene selectively up-regulated during the maintenance phase of LTP. AKAP-150 mRNA has a biphasic modulation in the dentate gyrus following the induction of LTP. The expression of AKAP-150 was 29% lower than stimulated controls 1 h after the induction of LTP. Its expression was enhanced 3 (50%), 6 (239%) and 12 h (210%) after induction, returning to control levels by 24 h postinduction. The NMDA receptor antagonist CPP blocked the tetanus-induced modulation of AKAP-150 expression. Interestingly, strong generalized stimulation produced by electroconvulsive shock did not increase the expression of AKAP-150. This implies that the AKAP-150 harbours a novel property of selective responsiveness to the stimulation patterns that trigger NMDA-dependent LTP in vivo. Its selective up-regulation during LTP and its identified functions as a scaffold for protein kinase A, protein kinase C, calmodulin, calcineurin and ionotropic glutamate receptors suggest that AKAP-150 encodes is an important effector protein in the expression of late LTP.
0953-816X
331-334
Génin, A.
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French, P.
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Doyère, V.
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Davis, S.
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Errington, M.L.
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Maroun, M.
aac05486-81c9-478e-b2ca-76809ca21fc4
Stean, T.
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Truchet, B.
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Webber, M.
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Wills, T.
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Richter-Levin, G.
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Sanger, G.
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Hunt, S.P.
c5e7e36d-af01-4fd1-8793-6ee6cf8c4e38
Mallet, J.
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Laroche, S.
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Bliss, T.V.P.
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O'Connor, V.
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Génin, A.
b37f9a9f-fea2-487a-a0b5-b782211d668b
French, P.
5edf2039-f680-4474-b826-78ed87b4fe00
Doyère, V.
9715c5c4-20f4-4a35-8d2a-665c95438cb4
Davis, S.
62413db4-dace-4e95-a731-51d916b8ff0d
Errington, M.L.
906d7e8f-e549-47b2-bd7f-3478167330cf
Maroun, M.
aac05486-81c9-478e-b2ca-76809ca21fc4
Stean, T.
499d15aa-d2de-43d3-a1a6-05f76dabed8d
Truchet, B.
4ee7a17e-b875-43f2-a6db-9530dec29f41
Webber, M.
241b817b-4d2c-4572-bf23-e1db92080ecf
Wills, T.
d9bc2190-f6cf-4007-bd42-2c1be6acdee8
Richter-Levin, G.
fa0ebf9c-4de6-4c23-bf2a-2a38c6ebee6f
Sanger, G.
cd3433c9-3326-417e-8aab-f8cbf75950d4
Hunt, S.P.
c5e7e36d-af01-4fd1-8793-6ee6cf8c4e38
Mallet, J.
c95710a0-c745-4855-9bfa-b438e75b5d40
Laroche, S.
53cde5d0-89e3-406b-b280-d0e11b044b2a
Bliss, T.V.P.
a448eacf-9563-4c65-93b0-a967ac4f3d30
O'Connor, V.
8021b06c-01a0-4925-9dde-a61c8fe278ca

Génin, A., French, P., Doyère, V., Davis, S., Errington, M.L., Maroun, M., Stean, T., Truchet, B., Webber, M., Wills, T., Richter-Levin, G., Sanger, G., Hunt, S.P., Mallet, J., Laroche, S., Bliss, T.V.P. and O'Connor, V. (2003) LTP but not seizure is associated with up-regulation of AKAP-150. European Journal of Neuroscience, 17 (2), 331-334. (doi:10.1046/j.1460-9568.2003.02462.x).

Record type: Article

Abstract

We have used differential display to profile and compare the mRNAs expressed in the hippocampus of freely moving animals after the induction of long-term potentiation (LTP) at the perforant path–dentate gyrus synapse with control rats receiving low-frequency stimulation. We have combined this with in situ hybridization and have identified A-kinase anchoring protein of 150 kDa (AKAP-150) as a gene selectively up-regulated during the maintenance phase of LTP. AKAP-150 mRNA has a biphasic modulation in the dentate gyrus following the induction of LTP. The expression of AKAP-150 was 29% lower than stimulated controls 1 h after the induction of LTP. Its expression was enhanced 3 (50%), 6 (239%) and 12 h (210%) after induction, returning to control levels by 24 h postinduction. The NMDA receptor antagonist CPP blocked the tetanus-induced modulation of AKAP-150 expression. Interestingly, strong generalized stimulation produced by electroconvulsive shock did not increase the expression of AKAP-150. This implies that the AKAP-150 harbours a novel property of selective responsiveness to the stimulation patterns that trigger NMDA-dependent LTP in vivo. Its selective up-regulation during LTP and its identified functions as a scaffold for protein kinase A, protein kinase C, calmodulin, calcineurin and ionotropic glutamate receptors suggest that AKAP-150 encodes is an important effector protein in the expression of late LTP.

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Published date: January 2003

Identifiers

Local EPrints ID: 24585
URI: http://eprints.soton.ac.uk/id/eprint/24585
ISSN: 0953-816X
PURE UUID: 24ac204a-6bf4-4288-b45b-94e05659d5e9
ORCID for V. O'Connor: ORCID iD orcid.org/0000-0003-3185-5709

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Date deposited: 31 Mar 2006
Last modified: 16 Mar 2024 03:13

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Contributors

Author: A. Génin
Author: P. French
Author: V. Doyère
Author: S. Davis
Author: M.L. Errington
Author: M. Maroun
Author: T. Stean
Author: B. Truchet
Author: M. Webber
Author: T. Wills
Author: G. Richter-Levin
Author: G. Sanger
Author: S.P. Hunt
Author: J. Mallet
Author: S. Laroche
Author: T.V.P. Bliss
Author: V. O'Connor ORCID iD

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