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Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover

Record type: Article

BACKGROUND: IVF is limited by low success rates and an unacceptably high multiple pregnancy rate. These outcomes would be improved significantly if a single embryo of high viability could be replaced in each treatment cycle, but widespread acceptance of such a policy is hindered by the lack of predictive factors for embryo selection. We have conducted a retrospective clinical study of a novel non-invasive method of embryo selection based on the depletion/appearance of amino acids in the culture medium. METHODS: Fifty-three cycles of IVF treatment using ICSI were studied. Embryos were cultured for 24 h in 4 µl drops of medium containing a physiological mixture of 18 amino acids. The spent medium was analysed for amino acid content by high performance liquid chromatography. RESULTS: The turnover of three amino acids, Asn, Gly and Leu, was significantly correlated with a clinical pregnancy and live birth. These correlations were independent of known predictors, such as female age, basal levels of FSH, embryo cell number and embryo morphological grade. CONCLUSIONS: Non-invasive assay of amino acid turnover has the potential to improve significantly the prospective selection of the most viable embryos, or single embryo, for replacement in an IVF cycle.

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Citation

Brison, D.R., Houghton, F.D., Falconer, D., Roberts, S.A., Hawkhead, J., Humpherson, P.G., Lieberman, B.A. and Leese, H.J. (2004) Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover Human Reproduction, 19, (10), pp. 2319-2324. (doi:10.1093/humrep/deh409).

More information

Published date: 2004
Keywords: amino acid, developmental potential, human preimplatation embryo selection, ivf

Identifiers

Local EPrints ID: 24639
URI: http://eprints.soton.ac.uk/id/eprint/24639
PURE UUID: efd83d52-d498-4480-b617-ad2128c5a135

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Date deposited: 04 Apr 2006
Last modified: 17 Jul 2017 16:13

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Contributors

Author: D.R. Brison
Author: F.D. Houghton
Author: D. Falconer
Author: S.A. Roberts
Author: J. Hawkhead
Author: P.G. Humpherson
Author: B.A. Lieberman
Author: H.J. Leese

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