Array painting: a method for the rapid analysis of aberrant chromosomes using DNA microarrays
Array painting: a method for the rapid analysis of aberrant chromosomes using DNA microarrays
Objective:
The authors describe a method, termed array painting, which allows the rapid, high resolution analysis of the content and breakpoints of aberrant chromosomes.
Methods:
Array painting is similar in concept to reverse chromosome painting and involves the hybridisation of probes generated by PCR of small numbers of flow sorted chromosomes on large insert genomic clone DNA microarrays.
Results and Conclusions:
By analysing patients with cytogenetically balanced chromosome rearrangements, the authors show the effectiveness of array painting as a method to map breakpoints prior to cloning and sequencing chromosome rearrangements.
chromosome rearrangement, DNA microarrays, reverse chromosome painting, chromosome breakpoint
664-670
Fiegler, H.
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Gribble, S.M.
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Burford, D.C.
d23a3e93-a89a-4a5d-ac37-00970dd34700
Carr, P.
f3736866-2f05-44ed-be6c-3be6bae4ee6c
Prigmore, E.
927d327c-f0f6-4e37-88f8-47de5a8e5a82
Porter, K.M.
060a1290-e449-4043-a701-a79508a677f7
Clegg, S.
f50afd3b-91b3-4aa8-b43c-b08e401f8d74
Crolla, J.A.
c5f23751-8de9-4a55-9cc5-ca2fb635769c
Dennis, N.R.
154aa617-52e2-4711-98ef-89fef8610de7
Jacobs, P.
44a49fb2-53e9-428e-a20c-373b75e457f1
Carter, N.P.
d9377125-5da4-45eb-bfbf-4e797d1c048a
2003
Fiegler, H.
7291cb06-165f-4371-bc96-869a52b29318
Gribble, S.M.
01b3b3bd-746f-4815-96ec-b8cb2c772554
Burford, D.C.
d23a3e93-a89a-4a5d-ac37-00970dd34700
Carr, P.
f3736866-2f05-44ed-be6c-3be6bae4ee6c
Prigmore, E.
927d327c-f0f6-4e37-88f8-47de5a8e5a82
Porter, K.M.
060a1290-e449-4043-a701-a79508a677f7
Clegg, S.
f50afd3b-91b3-4aa8-b43c-b08e401f8d74
Crolla, J.A.
c5f23751-8de9-4a55-9cc5-ca2fb635769c
Dennis, N.R.
154aa617-52e2-4711-98ef-89fef8610de7
Jacobs, P.
44a49fb2-53e9-428e-a20c-373b75e457f1
Carter, N.P.
d9377125-5da4-45eb-bfbf-4e797d1c048a
Fiegler, H., Gribble, S.M., Burford, D.C., Carr, P., Prigmore, E., Porter, K.M., Clegg, S., Crolla, J.A., Dennis, N.R., Jacobs, P. and Carter, N.P.
(2003)
Array painting: a method for the rapid analysis of aberrant chromosomes using DNA microarrays.
Journal of Medical Genetics, 40 (9), .
(doi:10.1136/jmg.40.9.664).
Abstract
Objective:
The authors describe a method, termed array painting, which allows the rapid, high resolution analysis of the content and breakpoints of aberrant chromosomes.
Methods:
Array painting is similar in concept to reverse chromosome painting and involves the hybridisation of probes generated by PCR of small numbers of flow sorted chromosomes on large insert genomic clone DNA microarrays.
Results and Conclusions:
By analysing patients with cytogenetically balanced chromosome rearrangements, the authors show the effectiveness of array painting as a method to map breakpoints prior to cloning and sequencing chromosome rearrangements.
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More information
Published date: 2003
Keywords:
chromosome rearrangement, DNA microarrays, reverse chromosome painting, chromosome breakpoint
Identifiers
Local EPrints ID: 24698
URI: http://eprints.soton.ac.uk/id/eprint/24698
ISSN: 0022-2593
PURE UUID: 1acbd9fb-7f17-463b-84ad-c8b8b09bcfcd
Catalogue record
Date deposited: 03 Apr 2006
Last modified: 15 Mar 2024 06:57
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Contributors
Author:
H. Fiegler
Author:
S.M. Gribble
Author:
D.C. Burford
Author:
P. Carr
Author:
E. Prigmore
Author:
K.M. Porter
Author:
S. Clegg
Author:
J.A. Crolla
Author:
N.R. Dennis
Author:
P. Jacobs
Author:
N.P. Carter
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