Microarray analysis of peroxisome proliferator-activated receptor-? induced changes in gene expression in macrophages
Microarray analysis of peroxisome proliferator-activated receptor-? induced changes in gene expression in macrophages
We used a combination of expression microarray and Northern blot analyses to identify target genes for peroxisome proliferator-activated receptor (PPAR) ? in RAW264.7 macrophages. PPAR? natural ligand 15-deoxy-?12,14 prostaglandin and synthetic ligands ciglitazone and rosiglitazone increased the expression of scavenger receptor CD36 and ATP-binding cassette transporter A1, as well as adipophilin (a lipid droplet coating protein involved in intracellular lipid storage and transport), calpain (a protease implicated in ABCA1 protein degradation), and ADAM8 (a disintegrin and metalloprotease protein involved in cell adhesion). These findings are relevant to understanding the effect of PPAR? activation on gene expression and cognate pathways in macrophages.
peroxisome proliferator, activated receptor-?, macrophages, gene expression, microarray analysis
505-510
Hodgkinson, Conrad P.
292cc7c3-b378-4911-8353-9a8c08a1c737
Ye, Shu
132b6474-1927-4f93-80db-2c620a31c1ab
2003
Hodgkinson, Conrad P.
292cc7c3-b378-4911-8353-9a8c08a1c737
Ye, Shu
132b6474-1927-4f93-80db-2c620a31c1ab
Hodgkinson, Conrad P. and Ye, Shu
(2003)
Microarray analysis of peroxisome proliferator-activated receptor-? induced changes in gene expression in macrophages.
Biochemical and Biophysical Research Communications, 308 (3), .
(doi:10.1016/S0006-291X(03)01416-5).
Abstract
We used a combination of expression microarray and Northern blot analyses to identify target genes for peroxisome proliferator-activated receptor (PPAR) ? in RAW264.7 macrophages. PPAR? natural ligand 15-deoxy-?12,14 prostaglandin and synthetic ligands ciglitazone and rosiglitazone increased the expression of scavenger receptor CD36 and ATP-binding cassette transporter A1, as well as adipophilin (a lipid droplet coating protein involved in intracellular lipid storage and transport), calpain (a protease implicated in ABCA1 protein degradation), and ADAM8 (a disintegrin and metalloprotease protein involved in cell adhesion). These findings are relevant to understanding the effect of PPAR? activation on gene expression and cognate pathways in macrophages.
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Published date: 2003
Keywords:
peroxisome proliferator, activated receptor-?, macrophages, gene expression, microarray analysis
Identifiers
Local EPrints ID: 24753
URI: http://eprints.soton.ac.uk/id/eprint/24753
ISSN: 0006-291X
PURE UUID: 90396a5a-cf76-4444-840a-825d48136991
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Date deposited: 05 Apr 2006
Last modified: 15 Mar 2024 06:58
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Contributors
Author:
Conrad P. Hodgkinson
Author:
Shu Ye
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