Simultaneous measurement of intracellular nitric oxide and free calcium levels in chordate eggs demonstrates that nitric oxide has no role at fertilization
Simultaneous measurement of intracellular nitric oxide and free calcium levels in chordate eggs demonstrates that nitric oxide has no role at fertilization
At fertilization in sea urchin, the free radical nitric oxide (NO) has recently been suggested to cause the intracellular Ca2+ rise responsible for egg activation. The authors suggested that NO could be a universal activator of eggs and the present study was set up to test this hypothesis. Intracellular NO and Ca2+ levels were monitored simultaneously in eggs of the mouse or the urochordate ascidian Ascidiella aspersa. Eggs were either fertilized or sperm extracts microinjected. Sperm-induced Ca2+ rises were not associated with any global, or local, change in intracellular NO, although we were able to detect NO produced by the addition of a NO donor. Furthermore, the NO synthase inhibitor NG-nitro--arginine methyl ester had no effect on sperm-induced Ca2+ release but did block completely ionomycin-induced NO synthase activation. Therefore, we suggest that the current data provide evidence that NO has no role in the fertilization of these two chordate eggs.
egg, fertilization, nitric oxide, calcium, mouse, mammal, ascidian, sea urchin
216-230
Hyslop, Louise A.
53165998-6cf8-42ad-bff8-9cc2522a11e6
Carroll, Michael
527c3628-58dc-4502-98e6-d03983beac9e
Nixon, Victoria L.
8dbba95a-2268-4413-b390-bff9d6e111e4
McDougall, Alex
23f6b2c7-b5fe-42b7-bf07-82e5a707e13a
Jones, Keith T.
7688cb4b-2f73-4b66-ac9e-db2d079fd21f
2001
Hyslop, Louise A.
53165998-6cf8-42ad-bff8-9cc2522a11e6
Carroll, Michael
527c3628-58dc-4502-98e6-d03983beac9e
Nixon, Victoria L.
8dbba95a-2268-4413-b390-bff9d6e111e4
McDougall, Alex
23f6b2c7-b5fe-42b7-bf07-82e5a707e13a
Jones, Keith T.
7688cb4b-2f73-4b66-ac9e-db2d079fd21f
Hyslop, Louise A., Carroll, Michael, Nixon, Victoria L., McDougall, Alex and Jones, Keith T.
(2001)
Simultaneous measurement of intracellular nitric oxide and free calcium levels in chordate eggs demonstrates that nitric oxide has no role at fertilization.
Developmental Biology, 234 (1), .
(doi:10.1006/dbio.2001.0252).
Abstract
At fertilization in sea urchin, the free radical nitric oxide (NO) has recently been suggested to cause the intracellular Ca2+ rise responsible for egg activation. The authors suggested that NO could be a universal activator of eggs and the present study was set up to test this hypothesis. Intracellular NO and Ca2+ levels were monitored simultaneously in eggs of the mouse or the urochordate ascidian Ascidiella aspersa. Eggs were either fertilized or sperm extracts microinjected. Sperm-induced Ca2+ rises were not associated with any global, or local, change in intracellular NO, although we were able to detect NO produced by the addition of a NO donor. Furthermore, the NO synthase inhibitor NG-nitro--arginine methyl ester had no effect on sperm-induced Ca2+ release but did block completely ionomycin-induced NO synthase activation. Therefore, we suggest that the current data provide evidence that NO has no role in the fertilization of these two chordate eggs.
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Published date: 2001
Keywords:
egg, fertilization, nitric oxide, calcium, mouse, mammal, ascidian, sea urchin
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Local EPrints ID: 24775
URI: http://eprints.soton.ac.uk/id/eprint/24775
ISSN: 0012-1606
PURE UUID: 359ecbe8-fdcf-440b-8a7e-6eb659f38751
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Date deposited: 04 Apr 2006
Last modified: 15 Mar 2024 06:58
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Contributors
Author:
Louise A. Hyslop
Author:
Michael Carroll
Author:
Victoria L. Nixon
Author:
Alex McDougall
Author:
Keith T. Jones
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