Headspace solid-phase microextraction profiling of volatile compounds in urine: application to metabolic investigations
Headspace solid-phase microextraction profiling of volatile compounds in urine: application to metabolic investigations
Volatile compounds contribute substantially to the metabolic pool in man. Their analysis in body fluids is problematic. We investigated headspace solid-phase microextraction (HS-SPME) with Carboxen–polydimethylsiloxane fibres and gas chromatography–mass spectrometry for profiling urinary volatile components. These fibres were more sensitive for very volatile and sulfur compounds than three other phases tested. We detected a wide range of compounds in normal urine at acid and alkaline pH. Profiles presented for five individuals with metabolic disturbances demonstrate abnormal accumulation of sulfur compounds, fatty acids and plasticisers. HS-SPME can complement profiling of non-volatile compounds in metabolic investigations and could be a useful extension of the diagnostic repertoire.
headspace solid-phase, microextraction, metabolism, volatile compounds
259-268
Mills, Graham A.
3f99eb9e-1c6c-44ec-ac67-361a6421830a
Walker, Valerie
e42b352f-5bdd-4ee0-adcd-0ce0fca21a71
2001
Mills, Graham A.
3f99eb9e-1c6c-44ec-ac67-361a6421830a
Walker, Valerie
e42b352f-5bdd-4ee0-adcd-0ce0fca21a71
Mills, Graham A. and Walker, Valerie
(2001)
Headspace solid-phase microextraction profiling of volatile compounds in urine: application to metabolic investigations.
Journal of Chromatography B: Biomedical Sciences and Applications, 753 (2), .
(doi:10.1016/S0378-4347(00)00554-5).
Abstract
Volatile compounds contribute substantially to the metabolic pool in man. Their analysis in body fluids is problematic. We investigated headspace solid-phase microextraction (HS-SPME) with Carboxen–polydimethylsiloxane fibres and gas chromatography–mass spectrometry for profiling urinary volatile components. These fibres were more sensitive for very volatile and sulfur compounds than three other phases tested. We detected a wide range of compounds in normal urine at acid and alkaline pH. Profiles presented for five individuals with metabolic disturbances demonstrate abnormal accumulation of sulfur compounds, fatty acids and plasticisers. HS-SPME can complement profiling of non-volatile compounds in metabolic investigations and could be a useful extension of the diagnostic repertoire.
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Published date: 2001
Keywords:
headspace solid-phase, microextraction, metabolism, volatile compounds
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Local EPrints ID: 24865
URI: http://eprints.soton.ac.uk/id/eprint/24865
PURE UUID: 018c01af-47cf-4cac-98af-a66339580a92
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Date deposited: 05 Apr 2006
Last modified: 15 Mar 2024 06:58
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Author:
Graham A. Mills
Author:
Valerie Walker
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