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Beta cell differentiation during early human pancreas development

Beta cell differentiation during early human pancreas development
Beta cell differentiation during early human pancreas development
Understanding gene expression profiles during early human pancreas development is limited by comparison to studies in rodents. In this study, from the inception of pancreatic formation, embryonic pancreatic epithelial cells, approximately half of which were proliferative, expressed nuclear PDX1 and cytoplasmic CK19. Later, in the fetal pancreas, insulin was the most abundant hormone detected during the first trimester in largely non-proliferative cells. At sequential stages of early fetal development, as the number of insulin-positive cell clusters increased, the detection of CK19 in these cells diminished. PDX1 remained expressed in fetal beta cells. Vascular structures were present within the loose stroma surrounding pancreatic epithelial cells during embryogenesis. At 10 weeks post-conception (w.p.c.), all clusters containing more than ten insulin-positive cells had developed an intimate relationship with these vessels, compared with the remainder of the developing pancreas. At 12-13 w.p.c., human fetal islets, penetrated by vasculature, contained cells independently immunoreactive for insulin, glucagon, somatostatin and pancreatic polypeptide (PP), coincident with the expression of maturity markers prohormone convertase 1/3 (PC1/3), islet amyloid polypeptide, Chromogranin A and, more weakly, GLUT2. These data support the function of fetal beta cells as true endocrine cells by the end of the first trimester of human pregnancy.
11-23
Piper, K.
a05b9b5d-f3af-42f0-8cdf-421b79127a89
Brickwood, S.
b11affc9-7fc8-4e45-8529-c39766afb522
Turnpenny, L.W.
c76a6b0c-5041-49be-ace2-9babcc85bd1e
Cameron, I.T.
f7595539-efa6-4687-b161-e1e93ff710f2
Ball, S.G.
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Wilson, D.I.
1500fca1-7082-4271-95f4-691f1d1252a2
Hanley, N.A.
ac5bcdcc-51dd-4f2c-ad06-fef6c1d50ebd
Piper, K.
a05b9b5d-f3af-42f0-8cdf-421b79127a89
Brickwood, S.
b11affc9-7fc8-4e45-8529-c39766afb522
Turnpenny, L.W.
c76a6b0c-5041-49be-ace2-9babcc85bd1e
Cameron, I.T.
f7595539-efa6-4687-b161-e1e93ff710f2
Ball, S.G.
001167e7-c620-41a6-b24c-63099ced52c4
Wilson, D.I.
1500fca1-7082-4271-95f4-691f1d1252a2
Hanley, N.A.
ac5bcdcc-51dd-4f2c-ad06-fef6c1d50ebd

Piper, K., Brickwood, S., Turnpenny, L.W., Cameron, I.T., Ball, S.G., Wilson, D.I. and Hanley, N.A. (2004) Beta cell differentiation during early human pancreas development. Journal of Endocrinology, 181 (1), 11-23. (doi:10.1677/joe.0.1810011).

Record type: Article

Abstract

Understanding gene expression profiles during early human pancreas development is limited by comparison to studies in rodents. In this study, from the inception of pancreatic formation, embryonic pancreatic epithelial cells, approximately half of which were proliferative, expressed nuclear PDX1 and cytoplasmic CK19. Later, in the fetal pancreas, insulin was the most abundant hormone detected during the first trimester in largely non-proliferative cells. At sequential stages of early fetal development, as the number of insulin-positive cell clusters increased, the detection of CK19 in these cells diminished. PDX1 remained expressed in fetal beta cells. Vascular structures were present within the loose stroma surrounding pancreatic epithelial cells during embryogenesis. At 10 weeks post-conception (w.p.c.), all clusters containing more than ten insulin-positive cells had developed an intimate relationship with these vessels, compared with the remainder of the developing pancreas. At 12-13 w.p.c., human fetal islets, penetrated by vasculature, contained cells independently immunoreactive for insulin, glucagon, somatostatin and pancreatic polypeptide (PP), coincident with the expression of maturity markers prohormone convertase 1/3 (PC1/3), islet amyloid polypeptide, Chromogranin A and, more weakly, GLUT2. These data support the function of fetal beta cells as true endocrine cells by the end of the first trimester of human pregnancy.

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Published date: 1 April 2004

Identifiers

Local EPrints ID: 24905
URI: http://eprints.soton.ac.uk/id/eprint/24905
PURE UUID: bb56a9f3-c73b-4936-ab85-4c9f8bbb8cec
ORCID for I.T. Cameron: ORCID iD orcid.org/0000-0002-4875-267X

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Date deposited: 04 Apr 2006
Last modified: 16 Mar 2024 03:00

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Contributors

Author: K. Piper
Author: S. Brickwood
Author: L.W. Turnpenny
Author: I.T. Cameron ORCID iD
Author: S.G. Ball
Author: D.I. Wilson
Author: N.A. Hanley

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