Ovarian modulators of type 1 11?-hydroxysteroid dehydrogenase (11?HSD) activity and intra-follicular cortisol:cortisone ratios correlate with the clinical outcome of IVF
Ovarian modulators of type 1 11?-hydroxysteroid dehydrogenase (11?HSD) activity and intra-follicular cortisol:cortisone ratios correlate with the clinical outcome of IVF
Background: Follicular fluid (FF) contains compounds that can modulate NADP+-dependent oxidation of cortisol by type 1 11?-hydroxysteroid dehydrogenase (11?HSD). The objective of this study was to investigate the relationships between levels of the ovarian modulators of type 1 11?HSD, intra-follicular cortisol:cortisone ratios and the clinical outcome of IVF cycles.
Methods: A single random sample of FF was aspirated from each of 132 patients undergoing gonadotrophin-stimulated IVF. Components of FF, resolved using C18 column chromatography, were evaluated for effects on NADP+-dependent cortisol oxidation in rat kidney homogenates. Intra- follicular steroid concentrations were measured by radioimmunoassays. Clinical pregnancies were confirmed by ultrasonography at 6 weeks post-embryo transfer.
Results: Levels of the hydrophilic ovarian 11?HSD stimuli were significantly lower (P < 0.0001) and levels of the hydrophobic ovarian 11?HSD inhibitors were significantly higher (P < 0.002) in conception versus non-conception cycles. Intra-follicular cortisol:cortisone ratios increased with the degree of inhibition of 11?HSD by the hydrophobic FF fractions. FF obtained from conception cycles had significantly higher cortisol:cortisone ratios than samples from non-conception cycles (12.9 ± 0.3 versus 8.5 ± 0.2, respectively; P < 0.0001).
Conclusions: Conception by IVF is associated with elevated intra-follicular cortisol:cortisone ratios, which reflect low levels of ovarian stimuli and/or high levels of ovarian inhibitors of type 1 11?HSD.
biology, ovary, molecular biology, humans, adult, activity, non-u.s.gov't, london, in vitro, pregnancy, animals, 11-beta-hydroxysteroid dehydrogenases, oxidation-reduction, methods
1603-1612
Thurston, L.M.
7fbe75fa-0164-4b3e-8ea5-0e4b63ea5332
Norgate, D.P.
96406c86-5b6e-485d-be2f-5e8cba906349
Jonas, K.C.
cf59567e-34ac-4d0b-a3dd-0569b92336ed
Gregory, L.
30c019cc-a300-40c8-b344-4d4f289ea90f
Wood, P.J.
f0dfe718-fa0f-43b1-9b2d-4bdc9c41320a
Cooke, B.A.
05550c39-438a-4f4f-aab1-20d72848a003
Michael, A.E.
a10d8c8e-8703-46cf-a490-3484cdc9d9dd
2003
Thurston, L.M.
7fbe75fa-0164-4b3e-8ea5-0e4b63ea5332
Norgate, D.P.
96406c86-5b6e-485d-be2f-5e8cba906349
Jonas, K.C.
cf59567e-34ac-4d0b-a3dd-0569b92336ed
Gregory, L.
30c019cc-a300-40c8-b344-4d4f289ea90f
Wood, P.J.
f0dfe718-fa0f-43b1-9b2d-4bdc9c41320a
Cooke, B.A.
05550c39-438a-4f4f-aab1-20d72848a003
Michael, A.E.
a10d8c8e-8703-46cf-a490-3484cdc9d9dd
Thurston, L.M., Norgate, D.P., Jonas, K.C., Gregory, L., Wood, P.J., Cooke, B.A. and Michael, A.E.
(2003)
Ovarian modulators of type 1 11?-hydroxysteroid dehydrogenase (11?HSD) activity and intra-follicular cortisol:cortisone ratios correlate with the clinical outcome of IVF.
Human Reproduction, 18 (8), .
(doi:10.1093/humrep/deg322).
(PMID:12871869)
Abstract
Background: Follicular fluid (FF) contains compounds that can modulate NADP+-dependent oxidation of cortisol by type 1 11?-hydroxysteroid dehydrogenase (11?HSD). The objective of this study was to investigate the relationships between levels of the ovarian modulators of type 1 11?HSD, intra-follicular cortisol:cortisone ratios and the clinical outcome of IVF cycles.
Methods: A single random sample of FF was aspirated from each of 132 patients undergoing gonadotrophin-stimulated IVF. Components of FF, resolved using C18 column chromatography, were evaluated for effects on NADP+-dependent cortisol oxidation in rat kidney homogenates. Intra- follicular steroid concentrations were measured by radioimmunoassays. Clinical pregnancies were confirmed by ultrasonography at 6 weeks post-embryo transfer.
Results: Levels of the hydrophilic ovarian 11?HSD stimuli were significantly lower (P < 0.0001) and levels of the hydrophobic ovarian 11?HSD inhibitors were significantly higher (P < 0.002) in conception versus non-conception cycles. Intra-follicular cortisol:cortisone ratios increased with the degree of inhibition of 11?HSD by the hydrophobic FF fractions. FF obtained from conception cycles had significantly higher cortisol:cortisone ratios than samples from non-conception cycles (12.9 ± 0.3 versus 8.5 ± 0.2, respectively; P < 0.0001).
Conclusions: Conception by IVF is associated with elevated intra-follicular cortisol:cortisone ratios, which reflect low levels of ovarian stimuli and/or high levels of ovarian inhibitors of type 1 11?HSD.
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Accepted/In Press date: 1 May 2003
Published date: 2003
Keywords:
biology, ovary, molecular biology, humans, adult, activity, non-u.s.gov't, london, in vitro, pregnancy, animals, 11-beta-hydroxysteroid dehydrogenases, oxidation-reduction, methods
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Local EPrints ID: 24989
URI: http://eprints.soton.ac.uk/id/eprint/24989
PURE UUID: 35b88866-fb2b-47d8-bfe1-7f5952abec89
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Date deposited: 06 Apr 2006
Last modified: 15 Mar 2024 06:59
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Author:
L.M. Thurston
Author:
D.P. Norgate
Author:
K.C. Jonas
Author:
L. Gregory
Author:
P.J. Wood
Author:
B.A. Cooke
Author:
A.E. Michael
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