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Ovarian modulators of type 1 11?-hydroxysteroid dehydrogenase (11?HSD) activity and intra-follicular cortisol:cortisone ratios correlate with the clinical outcome of IVF

Thurston, L.M., Norgate, D.P., Jonas, K.C., Gregory, L., Wood, P.J., Cooke, B.A. and Michael, A.E. (2003) Ovarian modulators of type 1 11?-hydroxysteroid dehydrogenase (11?HSD) activity and intra-follicular cortisol:cortisone ratios correlate with the clinical outcome of IVF Human Reproduction, 18, (8), pp. 1603-1612. (doi:10.1093/humrep/deg322). (PMID:12871869).

Record type: Article

Abstract

Background: Follicular fluid (FF) contains compounds that can modulate NADP+-dependent oxidation of cortisol by type 1 11?-hydroxysteroid dehydrogenase (11?HSD). The objective of this study was to investigate the relationships between levels of the ovarian modulators of type 1 11?HSD, intra-follicular cortisol:cortisone ratios and the clinical outcome of IVF cycles.

Methods: A single random sample of FF was aspirated from each of 132 patients undergoing gonadotrophin-stimulated IVF. Components of FF, resolved using C18 column chromatography, were evaluated for effects on NADP+-dependent cortisol oxidation in rat kidney homogenates. Intra- follicular steroid concentrations were measured by radioimmunoassays. Clinical pregnancies were confirmed by ultrasonography at 6 weeks post-embryo transfer.

Results: Levels of the hydrophilic ovarian 11?HSD stimuli were significantly lower (P < 0.0001) and levels of the hydrophobic ovarian 11?HSD inhibitors were significantly higher (P < 0.002) in conception versus non-conception cycles. Intra-follicular cortisol:cortisone ratios increased with the degree of inhibition of 11?HSD by the hydrophobic FF fractions. FF obtained from conception cycles had significantly higher cortisol:cortisone ratios than samples from non-conception cycles (12.9 ± 0.3 versus 8.5 ± 0.2, respectively; P < 0.0001).

Conclusions: Conception by IVF is associated with elevated intra-follicular cortisol:cortisone ratios, which reflect low levels of ovarian stimuli and/or high levels of ovarian inhibitors of type 1 11?HSD.

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More information

Accepted/In Press date: 1 May 2003
Published date: 2003
Keywords: biology, ovary, molecular biology, humans, adult, activity, non-u.s.gov't, london, in vitro, pregnancy, animals, 11-beta-hydroxysteroid dehydrogenases, oxidation-reduction, methods

Identifiers

Local EPrints ID: 24989
URI: http://eprints.soton.ac.uk/id/eprint/24989
PURE UUID: 35b88866-fb2b-47d8-bfe1-7f5952abec89

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Date deposited: 06 Apr 2006
Last modified: 17 Jul 2017 16:12

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Contributors

Author: L.M. Thurston
Author: D.P. Norgate
Author: K.C. Jonas
Author: L. Gregory
Author: P.J. Wood
Author: B.A. Cooke
Author: A.E. Michael

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