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Solid-phase extraction in clinical biochemistry

Walker, Valerie and Mills, Graham A. (2002) Solid-phase extraction in clinical biochemistry Annals of Clinical Biochemistry, 39, (5), pp. 464-477. (doi:10.1258/000456302320314476).

Record type: Article


In order to measure low concentrations of analytes in plasma and urine, it is often necessary to extract and concentrate them. With solid-phase extraction (SPE), this is achieved by partitioning the analytes between a solid and a liquid or headspace vapour. A wide range of high-quality materials is now available to do this, offering a variety of separation modes for different applications. These include partitioning using reversed-phase, normal-phase, ion-exchange, restricted-access and immunoaffinity sorbents or molecularly imprinted polymers and, increasingly, combinations of these processes. Solid-phase microextraction was introduced to analyse volatile and semi-volatile compounds. The range of sampling formats has expanded from simple packed syringes to cartridges, disks, SPE pipette tips and 96-well plates. These developments have facilitated automated off- and on-line sample processing. The basic principles of SPE and the recent innovations are reviewed here. This is a technological growth area. Some of the developments are finding application in clinical toxicology. However, they could also be of wider value in clinical chemistry - for example, for analyses of volatile and non-volatile metabolites, peptides, radioactive elements and trace metal speciation.

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Published date: 1 September 2002


Local EPrints ID: 25024
ISSN: 0004-5632
PURE UUID: 10a2ee9d-ff59-4687-8f86-ca943ef407c3

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Date deposited: 06 Apr 2006
Last modified: 17 Jul 2017 16:12

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Author: Valerie Walker
Author: Graham A. Mills

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