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An efficient procedure for genotyping single nucleotide polymorphisms

An efficient procedure for genotyping single nucleotide polymorphisms
An efficient procedure for genotyping single nucleotide polymorphisms
Analysis of single nucleotide polymorphisms (SNPs) has been and will be increasingly utilized in various genetic disciplines, particularly in studying genetic determinants of complex diseases. Such studies will be facilitated by rapid, simple, low cost and high throughput methodologies for SNP genotyping. One such method is reported here, named tetra-primer ARMS-PCR, which employs two primer pairs to amplify, respectively, the two different alleles of a SNP in a single PCR reaction. A computer program for designing primers was developed. Tetra-primer ARMS-PCR was combined with microplate array diagonal gel electrophoresis, gaining the advantage of high throughput for gel-based resolution of tetra-primer ARMS-PCR products. The technique was applied to analyse a number of SNPs and the results were completely consistent with those from an independent method, restriction fragment length polymorphism analysis.
0305-1048
p.E88
Ye, Shu
132b6474-1927-4f93-80db-2c620a31c1ab
Dhillon, Sahar
61831788-a120-426d-92d0-f15b8425bb57
Ke, Xiayi
3d3489dc-c021-4669-bf5c-30c8733c1183
Collins, Andrew R.
7daa83eb-0b21-43b2-af1a-e38fb36e2a64
Day, Ian N.M.
b749b30a-1f4c-40eb-af0e-a50427388b39
Ye, Shu
132b6474-1927-4f93-80db-2c620a31c1ab
Dhillon, Sahar
61831788-a120-426d-92d0-f15b8425bb57
Ke, Xiayi
3d3489dc-c021-4669-bf5c-30c8733c1183
Collins, Andrew R.
7daa83eb-0b21-43b2-af1a-e38fb36e2a64
Day, Ian N.M.
b749b30a-1f4c-40eb-af0e-a50427388b39

Ye, Shu, Dhillon, Sahar, Ke, Xiayi, Collins, Andrew R. and Day, Ian N.M. (2001) An efficient procedure for genotyping single nucleotide polymorphisms. Nucleic Acids Research, 29 (17), p.E88.

Record type: Article

Abstract

Analysis of single nucleotide polymorphisms (SNPs) has been and will be increasingly utilized in various genetic disciplines, particularly in studying genetic determinants of complex diseases. Such studies will be facilitated by rapid, simple, low cost and high throughput methodologies for SNP genotyping. One such method is reported here, named tetra-primer ARMS-PCR, which employs two primer pairs to amplify, respectively, the two different alleles of a SNP in a single PCR reaction. A computer program for designing primers was developed. Tetra-primer ARMS-PCR was combined with microplate array diagonal gel electrophoresis, gaining the advantage of high throughput for gel-based resolution of tetra-primer ARMS-PCR products. The technique was applied to analyse a number of SNPs and the results were completely consistent with those from an independent method, restriction fragment length polymorphism analysis.

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Published date: 2001

Identifiers

Local EPrints ID: 25043
URI: http://eprints.soton.ac.uk/id/eprint/25043
ISSN: 0305-1048
PURE UUID: cf3449ba-7fbb-4eaf-a9a1-93fb8e99c5ef
ORCID for Andrew R. Collins: ORCID iD orcid.org/0000-0001-7108-0771

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Date deposited: 06 Apr 2006
Last modified: 28 Apr 2022 01:36

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Contributors

Author: Shu Ye
Author: Sahar Dhillon
Author: Xiayi Ke
Author: Ian N.M. Day

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