Effects of nociceptin and analogues of nociceptin upon spontaneous dorsal root activity recorded from an in vitro preparation of rat spinal cord
Effects of nociceptin and analogues of nociceptin upon spontaneous dorsal root activity recorded from an in vitro preparation of rat spinal cord
The 17 amino acid peptide nociceptin has been implicated in pain modulation in the central nervous system. The effects of bath applied
nociceptin, and some analogues of nociceptin, upon spontaneous lumbar dorsal root activity have been investigated in an isolated preparation
of rat spinal cord. Nociceptin was found to reversibly depress spontaneous dorsal root activity at concentrations of 1.0 mMand 10.0 mM(IC50
2.0 mM), whereas acetyl-nociceptin at concentrations up to 10 mMhad no detectable effect. Omission of the last four amino acids (nociceptin
1–13), increased the potency of the effect upon dorsal root activity by approximately 100-fold (IC50 30 nM), but activity was lost when only
the first seven amino acids of the nociceptin molecule (nociceptin 1–7) were tested.
nociceptin, spinal cord, primary afferent fibres, dorsal root reflex
190-192
Maile, R.A.
aaf8cd4b-5485-45f0-b98d-943f0c03a4da
Walker, R.J.
b6597591-587e-488a-8a54-89156c42ce8d
Sharma, R.P.
aef51420-fc90-49d8-b04c-142214c2961a
Bagust, J.
d746f6e0-788c-416f-8165-9a09eb8e08c9
3 October 2003
Maile, R.A.
aaf8cd4b-5485-45f0-b98d-943f0c03a4da
Walker, R.J.
b6597591-587e-488a-8a54-89156c42ce8d
Sharma, R.P.
aef51420-fc90-49d8-b04c-142214c2961a
Bagust, J.
d746f6e0-788c-416f-8165-9a09eb8e08c9
Maile, R.A., Walker, R.J., Sharma, R.P. and Bagust, J.
(2003)
Effects of nociceptin and analogues of nociceptin upon spontaneous dorsal root activity recorded from an in vitro preparation of rat spinal cord.
Neuroscience Letters, 350 (3), .
(doi:10.1016/S0304-3940(03)00910-8).
Abstract
The 17 amino acid peptide nociceptin has been implicated in pain modulation in the central nervous system. The effects of bath applied
nociceptin, and some analogues of nociceptin, upon spontaneous lumbar dorsal root activity have been investigated in an isolated preparation
of rat spinal cord. Nociceptin was found to reversibly depress spontaneous dorsal root activity at concentrations of 1.0 mMand 10.0 mM(IC50
2.0 mM), whereas acetyl-nociceptin at concentrations up to 10 mMhad no detectable effect. Omission of the last four amino acids (nociceptin
1–13), increased the potency of the effect upon dorsal root activity by approximately 100-fold (IC50 30 nM), but activity was lost when only
the first seven amino acids of the nociceptin molecule (nociceptin 1–7) were tested.
Text
sdarticle.pdf
- Other
Restricted to Registered users only
More information
Published date: 3 October 2003
Keywords:
nociceptin, spinal cord, primary afferent fibres, dorsal root reflex
Identifiers
Local EPrints ID: 25076
URI: http://eprints.soton.ac.uk/id/eprint/25076
ISSN: 0304-3940
PURE UUID: 4eb7cec0-94cb-401d-88a2-73c6fd2646f4
Catalogue record
Date deposited: 21 Apr 2006
Last modified: 15 Mar 2024 07:00
Export record
Altmetrics
Contributors
Author:
R.A. Maile
Author:
R.J. Walker
Author:
R.P. Sharma
Author:
J. Bagust
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics