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Effects of nociceptin and analogues of nociceptin upon spontaneous dorsal root activity recorded from an in vitro preparation of rat spinal cord

Effects of nociceptin and analogues of nociceptin upon spontaneous dorsal root activity recorded from an in vitro preparation of rat spinal cord
Effects of nociceptin and analogues of nociceptin upon spontaneous dorsal root activity recorded from an in vitro preparation of rat spinal cord
The 17 amino acid peptide nociceptin has been implicated in pain modulation in the central nervous system. The effects of bath applied nociceptin, and some analogues of nociceptin, upon spontaneous lumbar dorsal root activity have been investigated in an isolated preparation of rat spinal cord. Nociceptin was found to reversibly depress spontaneous dorsal root activity at concentrations of 1.0 mMand 10.0 mM(IC50 2.0 mM), whereas acetyl-nociceptin at concentrations up to 10 mMhad no detectable effect. Omission of the last four amino acids (nociceptin 1–13), increased the potency of the effect upon dorsal root activity by approximately 100-fold (IC50 30 nM), but activity was lost when only the first seven amino acids of the nociceptin molecule (nociceptin 1–7) were tested.
nociceptin, spinal cord, primary afferent fibres, dorsal root reflex
0304-3940
190-192
Maile, R.A.
aaf8cd4b-5485-45f0-b98d-943f0c03a4da
Walker, R.J.
b6597591-587e-488a-8a54-89156c42ce8d
Sharma, R.P.
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Bagust, J.
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Maile, R.A.
aaf8cd4b-5485-45f0-b98d-943f0c03a4da
Walker, R.J.
b6597591-587e-488a-8a54-89156c42ce8d
Sharma, R.P.
aef51420-fc90-49d8-b04c-142214c2961a
Bagust, J.
d746f6e0-788c-416f-8165-9a09eb8e08c9

Maile, R.A., Walker, R.J., Sharma, R.P. and Bagust, J. (2003) Effects of nociceptin and analogues of nociceptin upon spontaneous dorsal root activity recorded from an in vitro preparation of rat spinal cord. Neuroscience Letters, 350 (3), 190-192. (doi:10.1016/S0304-3940(03)00910-8).

Record type: Article

Abstract

The 17 amino acid peptide nociceptin has been implicated in pain modulation in the central nervous system. The effects of bath applied nociceptin, and some analogues of nociceptin, upon spontaneous lumbar dorsal root activity have been investigated in an isolated preparation of rat spinal cord. Nociceptin was found to reversibly depress spontaneous dorsal root activity at concentrations of 1.0 mMand 10.0 mM(IC50 2.0 mM), whereas acetyl-nociceptin at concentrations up to 10 mMhad no detectable effect. Omission of the last four amino acids (nociceptin 1–13), increased the potency of the effect upon dorsal root activity by approximately 100-fold (IC50 30 nM), but activity was lost when only the first seven amino acids of the nociceptin molecule (nociceptin 1–7) were tested.

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Published date: 3 October 2003
Keywords: nociceptin, spinal cord, primary afferent fibres, dorsal root reflex

Identifiers

Local EPrints ID: 25076
URI: http://eprints.soton.ac.uk/id/eprint/25076
ISSN: 0304-3940
PURE UUID: 4eb7cec0-94cb-401d-88a2-73c6fd2646f4

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Date deposited: 21 Apr 2006
Last modified: 15 Mar 2024 07:00

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Contributors

Author: R.A. Maile
Author: R.J. Walker
Author: R.P. Sharma
Author: J. Bagust

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