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The effect of graded levels of dietary casein, with or without methionine supplementation, on glutathione concentration in unstressed and endotoxin-treated rats

Alhamdan, A.A. and Grimble, R.F. (2003) The effect of graded levels of dietary casein, with or without methionine supplementation, on glutathione concentration in unstressed and endotoxin-treated rats International Journal for Vitamin and Nutrition Research, 73, (6), pp. 468-477.

Record type: Article

Abstract

Glutathione (GSH) concentration was measured in rats fed either graded levels of dietary casein (experiment 1; 180 g, 120 g, 80 g, or 60 g protein/kg diet) or graded levels of dietary casein, supplemented with methionine to equalize dietary sulfur amino acid content to that seen in an 180 g/kg casein diet supplemented with 0.3 g L-methionine/kg diet (experiment 2; 180 g protein +0.3 g L-methionine, 80 g protein +6.70 g L-methionine, or 60 g protein +7.45 g L-methionine/kg diet). Rats were given an inflammatory challenge by intraperitoneal injection of endotoxin (lipopolysaccharide from Escherichia coli), and were compared with ad libitum and pair-fed controls. Glutathione concentration in various organs (liver, lung, spleen, and thymus) decreased in animals fed the low-protein diets (80 g or 60 g/kg diet). Addition of the sulfur amino acid, methionine, to the low-protein diets restored glutathione concentrations in animals fed ad libitum and prevented the fall in GSH concentration, which occurred in lung, spleen, and thymus in response to the endotoxin. Despite the similarity in the amount of sulfur amino acid consumed between the groups fed the 180 g protein +0.3 g L-methionine and the 60 g protein +7.45 g L-methionine/kg diet, in experiment 2, hepatic GSH concentration significantly increased in the latter group, in animals fed ad libitum and in the endotoxin-treated animals, but not in the pair-fed controls.

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More information

Published date: 2003
Additional Information: Vitamin and Nutrition Research 6/2003
Keywords: methionine, glutathione, endotoxin, cysteine, dietary casein

Identifiers

Local EPrints ID: 25194
URI: http://eprints.soton.ac.uk/id/eprint/25194
ISSN: 0300-9831
PURE UUID: 243f98d8-8bfa-45a1-a8a9-ab3b956e3144

Catalogue record

Date deposited: 11 Apr 2006
Last modified: 17 Jul 2017 16:11

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Contributors

Author: A.A. Alhamdan
Author: R.F. Grimble

University divisions

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