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Selective synthesis of bone morphogenetic proteins-1, -3, -4 and bone sialoprotein may be important for osteoinduction by Saos-2 cells

Anderson, H. Clarke, Reynolds, Paul R., Hsu, Howard H.T., Missana, Lilliana, Masuhara, Kensaku, Moylan, Paul E. and Roach, Helmtrude I. (2002) Selective synthesis of bone morphogenetic proteins-1, -3, -4 and bone sialoprotein may be important for osteoinduction by Saos-2 cells Journal of Bone and Mineral Metabolism, 20, (2), pp. 73-82. (doi:10.1007/s007740200010).

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An ability to induce new bone formation at a required site would represent a considerable advance in bone repair and tissue engineering. It has been shown that the healing of critical-size bone defects in rats can be augmented by extracts of Saos-2 cells. These human osteosarcoma cells uniquely contain a bone-inducing activity, whereas other human osteosarcoma cells, e.g., U-2 OS cells, cannot replicate the osteoinductive capacity. To understand the necessary components of the Saos-2 bone-inducing activity, this study compared osteoinductive Saos-2 cells with non-osteoinductive U-2 OS cells with respect to the synthesis of bone morphogenetic proteins (BMPs)-1, -2, -3, -4, -5, -6, and -7 and the non-collagenous matrix proteins bone sialoprotein (BSP), osteonectin (ON), osteopontin (OPN), and osteocalcin (OC). The main differences were abundant synthesis of BMP-1/tolloid, BMP-3, -4, and BSP by Saos-2 cells, but absence or reduced synthesis in U-2 OS cells. BMP-2 and -7 were present in low amounts in both cell types, while BMP-5 and -6 were more abundant in U-2 OS cells, suggesting that these BMPs were of lesser importance for the osteoinductivity of Saos-2 cells. However, a relatively high expression of BMP-3 and -4, together with BMP-1/tolloid, may be important for the osteoinductive capacity of Saos-2 cells. The inability of U2-OS cells to induce bone, despite expressing most of the BMPs, may be due to an insufficiency of tolloid, BMP-3 or -4, BSP, and/or other unknown factors. A better understanding of the necessary components of the Saos-2 cell bone-inducing agent may, in future, lead to clinically useful Saos-2 cell products for bone repair and tissue engineering.

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Published date: March 2002
Keywords: bone morphogenetic proteins, bone sialopro-tein, osteoinduction, tolloid, saos-2 cells


Local EPrints ID: 25198
ISSN: 0914-8779
PURE UUID: bf65bbee-8d1d-46af-9b42-934699a8f75c

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Date deposited: 07 Apr 2006
Last modified: 17 Jul 2017 16:11

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Author: H. Clarke Anderson
Author: Paul R. Reynolds
Author: Howard H.T. Hsu
Author: Lilliana Missana
Author: Kensaku Masuhara
Author: Paul E. Moylan
Author: Helmtrude I. Roach

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