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Intermittent umbilical cord occlusion in the ovine fetus: effects on blood glucose, insulin, and glucagon and on pancreatic development

Record type: Article

Objective: to determine whether repetitive umbilical cord occlusion resulting in fetal hypoxemia but not cumulative acidosis also affects fetal glucose levels and the levels of the regulatory hormones insulin and glucagon, by altering glucose delivery and with repetitive insults by inducing fetal glucose production, thus possibly affecting pancreatic development.
Methods: fifteen chronically catheterized fetal sheep were studied over 21 days. Umbilical cord occlusions (UCOs) (duration 90 seconds) were performed every 30 minutes for 3–4 hours each day. Fetal arterial blood was sampled at predetermined times on days 1, 9, and 18 for blood gases, pH, glucose, lactate, insulin, and glucagon. When animals were sacrificed, fetal pancreatic tissues were collected for insulin immunostaining.
Results: blood glucose decreased acutely with each UCO but showed a cumulative increase of approximately 30% over the course of each sampling day. Although plasma insulin levels also increased over the course of sampling on days 9 and 18, plasma glucagon levels remained unchanged throughout the study. The percentage of pancreatic islet cells immunopositive for insulin, which averaged 67%, was also unchanged in experimental compared with control animals.
Conclusion: umbilical cord occlusion during the latter part of pregnancy, which caused severe but limited hypoxemia, also resulted in acute decreases in blood glucose levels because of reduced exogenous glucose delivery and a cumulative increase in glucose in response to repetitive insults, possibly by inducing fetal glucose production, enhancing glucose delivery, or both. However, repetitive UCO as studied had minimal effect on plasma insulin levels and no effect on glucagon levels or on pancreatic immunostaining for insulin, and thus had no evident effect on pancreatic development.

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Citation

Czikk, Marie J., Green, Lucy R., Kawagoe, Yasuyuki, McDonald, Thomas J., Hill, David J. and Richardson, Bryan S. (2001) Intermittent umbilical cord occlusion in the ovine fetus: effects on blood glucose, insulin, and glucagon and on pancreatic development Journal of the Society for Gynecologic Investigation, 8, (4), pp. 191-197. (doi:10.1016/S1071-5576(01)00114-9).

More information

Published date: 2001
Keywords: umbilical cord occlusion, fetal insulin and glucagon, pancreas

Identifiers

Local EPrints ID: 25391
URI: http://eprints.soton.ac.uk/id/eprint/25391
ISSN: 1071-5576
PURE UUID: 6a0999f4-9af5-4dc7-a83f-259628e91fb6
ORCID for Lucy R. Green: ORCID iD orcid.org/0000-0001-7423-9696

Catalogue record

Date deposited: 07 Apr 2006
Last modified: 17 Jul 2017 16:11

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Contributors

Author: Marie J. Czikk
Author: Lucy R. Green ORCID iD
Author: Yasuyuki Kawagoe
Author: Thomas J. McDonald
Author: David J. Hill
Author: Bryan S. Richardson

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