The end-product method of measuring whole-body protein turnover: a review of published results and a comparison with those obtained by leucine infusion
The end-product method of measuring whole-body protein turnover: a review of published results and a comparison with those obtained by leucine infusion
The present review summarizes the results of all published papers on whole-body protein turnover in man measured by [15N]glycine and the end-product method using both urea and ammonia. It begins with a short account of the underlying assumptions and the justification for the use of [15N]glycine. The results are then compared with those of a large sample of measurements by the 'gold standard' precursor method with continuous infusion of [13C]leucine. The pros and cons of the two methods are compared and it is suggested that there is a place for further work by the less invasive end-product method, particularly for population studies of the genetic, environmental and functional determinants of whole-body rates of protein synthesis.
protein turnover, [15N]Glycine, end product, leucine infusion
141-153
Duggleby, S.L.
2077aa39-da37-4932-98cb-e15a454ad6b3
Waterlow, J.C.
1a8f1da6-0be0-4007-8d44-8c141e5614fd
August 2005
Duggleby, S.L.
2077aa39-da37-4932-98cb-e15a454ad6b3
Waterlow, J.C.
1a8f1da6-0be0-4007-8d44-8c141e5614fd
Duggleby, S.L. and Waterlow, J.C.
(2005)
The end-product method of measuring whole-body protein turnover: a review of published results and a comparison with those obtained by leucine infusion.
British Journal of Nutrition, 94 (2), .
(doi:10.1079/BJN20051460).
Abstract
The present review summarizes the results of all published papers on whole-body protein turnover in man measured by [15N]glycine and the end-product method using both urea and ammonia. It begins with a short account of the underlying assumptions and the justification for the use of [15N]glycine. The results are then compared with those of a large sample of measurements by the 'gold standard' precursor method with continuous infusion of [13C]leucine. The pros and cons of the two methods are compared and it is suggested that there is a place for further work by the less invasive end-product method, particularly for population studies of the genetic, environmental and functional determinants of whole-body rates of protein synthesis.
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Published date: August 2005
Keywords:
protein turnover, [15N]Glycine, end product, leucine infusion
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Local EPrints ID: 25421
URI: http://eprints.soton.ac.uk/id/eprint/25421
ISSN: 0007-1145
PURE UUID: dfe3d9f4-6042-439d-a3b4-edeae0ff70b1
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Date deposited: 07 Apr 2006
Last modified: 15 Mar 2024 07:02
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Author:
S.L. Duggleby
Author:
J.C. Waterlow
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