The University of Southampton
University of Southampton Institutional Repository

Relative contribution of cell contact pattern, specific PKC isoforms and gap junctional communication in tight junction assembly in the mouse early embryo

Relative contribution of cell contact pattern, specific PKC isoforms and gap junctional communication in tight junction assembly in the mouse early embryo
Relative contribution of cell contact pattern, specific PKC isoforms and gap junctional communication in tight junction assembly in the mouse early embryo
In mouse early development, cell contact patterns regulate the spatial organization and segregation of inner cell mass (ICM) and trophectoderm epithelium (TE) during blastocyst morphogenesis. Progressive membrane assembly of tight junctional (TJ) proteins in the differentiating TE during cleavage is upregulated by cell contact asymmetry (outside position) and suppressed within the ICM by cell contact symmetry (inside position). This is reversible, and immunosurgical isolation of the ICM induces upregulation of TJ assembly in a sequence that broadly mimics that occurring during blastocyst formation. The mechanism relating cell contact pattern and TJ assembly was investigated in the ICM model with respect to PKC-mediated signaling and gap junctional communication. Our results indicate that complete cell contact asymmetry is required for TJ biogenesis and acts upstream of PKC-mediated signaling. Specific inhibition of two PKC isoforms, PKC? and ?, revealed that both PKC activities are required for membrane assembly of ZO-2 TJ protein, while only PKC? activity is involved in regulating ZO-1?+ membrane assembly, suggesting different mechanisms for individual TJ proteins. Gap junctional communication had no apparent influence on either TJ formation or PKC signaling but was itself affected by changes of cell contact patterns. Our data suggest that the dynamics of cell contact patterns coordinate the spatial organization of TJ formation via specific PKC signaling pathways during blastocyst biogenesis.
protein kinase C, mouse embryo, trophectoderm, tight junction, ZO-1, cell contact pattern, inner cell mass, ZO-2
0012-1606
234-247
Eckert, Judith J.
729bfa49-7053-458d-8e84-3e70e4d98e57
McCallum, Amanda
13df1cf2-9b9d-47fc-a184-1f768d429672
Mears, Andrew
36c51693-c476-4f3f-a5a0-bc18b935e38f
Rumsby, Martin G.
ffedf189-2943-4a8d-9b75-bcfb9fa8323c
Cameron, Iain T.
f7595539-efa6-4687-b161-e1e93ff710f2
Fleming, Tom P.
2abf761a-e5a1-4fa7-a2c8-12e32d5d4c03
Eckert, Judith J.
729bfa49-7053-458d-8e84-3e70e4d98e57
McCallum, Amanda
13df1cf2-9b9d-47fc-a184-1f768d429672
Mears, Andrew
36c51693-c476-4f3f-a5a0-bc18b935e38f
Rumsby, Martin G.
ffedf189-2943-4a8d-9b75-bcfb9fa8323c
Cameron, Iain T.
f7595539-efa6-4687-b161-e1e93ff710f2
Fleming, Tom P.
2abf761a-e5a1-4fa7-a2c8-12e32d5d4c03

Eckert, Judith J., McCallum, Amanda, Mears, Andrew, Rumsby, Martin G., Cameron, Iain T. and Fleming, Tom P. (2005) Relative contribution of cell contact pattern, specific PKC isoforms and gap junctional communication in tight junction assembly in the mouse early embryo. Developmental Biology, 288 (1), 234-247. (doi:10.1016/j.ydbio.2005.09.037).

Record type: Article

Abstract

In mouse early development, cell contact patterns regulate the spatial organization and segregation of inner cell mass (ICM) and trophectoderm epithelium (TE) during blastocyst morphogenesis. Progressive membrane assembly of tight junctional (TJ) proteins in the differentiating TE during cleavage is upregulated by cell contact asymmetry (outside position) and suppressed within the ICM by cell contact symmetry (inside position). This is reversible, and immunosurgical isolation of the ICM induces upregulation of TJ assembly in a sequence that broadly mimics that occurring during blastocyst formation. The mechanism relating cell contact pattern and TJ assembly was investigated in the ICM model with respect to PKC-mediated signaling and gap junctional communication. Our results indicate that complete cell contact asymmetry is required for TJ biogenesis and acts upstream of PKC-mediated signaling. Specific inhibition of two PKC isoforms, PKC? and ?, revealed that both PKC activities are required for membrane assembly of ZO-2 TJ protein, while only PKC? activity is involved in regulating ZO-1?+ membrane assembly, suggesting different mechanisms for individual TJ proteins. Gap junctional communication had no apparent influence on either TJ formation or PKC signaling but was itself affected by changes of cell contact patterns. Our data suggest that the dynamics of cell contact patterns coordinate the spatial organization of TJ formation via specific PKC signaling pathways during blastocyst biogenesis.

PDF
Eckert_et_al,_2005,_Dev_Biol_288,_234-247.pdf - Version of Record
Restricted to Registered users only
Download (733kB)
Request a copy

More information

Published date: 4 November 2005
Additional Information: All the work for this paper took place within my laboratory and funded by my grants (Tom Fleming)
Keywords: protein kinase C, mouse embryo, trophectoderm, tight junction, ZO-1, cell contact pattern, inner cell mass, ZO-2

Identifiers

Local EPrints ID: 25426
URI: https://eprints.soton.ac.uk/id/eprint/25426
ISSN: 0012-1606
PURE UUID: f5dc53af-4f3b-461a-b67e-f53332e3e037
ORCID for Iain T. Cameron: ORCID iD orcid.org/0000-0002-4875-267X

Catalogue record

Date deposited: 06 Apr 2006
Last modified: 06 Jun 2018 12:56

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×