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Regulation of dietary fatty acid entrapment in subcutaneous adipose tissue and skeletal muscle

Record type: Article

Using stable isotopic labeling of dietary fatty acids in conjunction with arteriovenous difference measurements, we have assessed the regulation of lipoprotein lipase-derived fatty acid entrapment in subcutaneous adipose tissue and forearm muscle in healthy subjects in the postprandial state. Eight volunteers fasted overnight and were then given a mixed meal containing [ 1-13C]palmitic acid and [1-13C]oleic acid. At baseline and for 6 h after the meal, blood samples were obtained from an arterialized hand vein and veins draining subcutaneous abdominal adipose tissue and forearm muscle, and arteriovenous differences were calculated.
Entrapment of labeled fatty acids released by circulating triacylglycerol hydrolysis was close to 100% at 60 min, decreasing to 10–30% by 360 min. Entrapment of labeled fatty acids in forearm muscle was >100% and did not change with time. This study shows that entrapment of dietary fatty acids in adipose tissue in the postprandial period is a highly regulated process (varying with time) and that this can be studied in humans using stable isotope- labeled fatty acids in combination with measurement of appropriate arteriovenous differences. Also, fatty acid trapping in skeletal muscle is fundamentally different from that in adipose tissue, in that all the fatty acids released by lipoprotein lipase in skeletal muscle are taken up by the tissue.

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Citation

Evans, Kevin, Burdge, Graham C., Wootton, Stephen A., Clark, Mo L. and Frayn, Keith N. (2002) Regulation of dietary fatty acid entrapment in subcutaneous adipose tissue and skeletal muscle Diabetes, 51, (9), pp. 2684-2690. (doi:10.2337/diabetes.51.9.2684).

More information

Published date: September 2002

Identifiers

Local EPrints ID: 25476
URI: http://eprints.soton.ac.uk/id/eprint/25476
ISSN: 0012-1797
PURE UUID: d1efcf82-d1e3-4f5b-8d08-b3838fd2bba3
ORCID for Graham C. Burdge: ORCID iD orcid.org/0000-0002-7665-2967

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Date deposited: 19 Apr 2006
Last modified: 17 Jul 2017 16:10

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Contributors

Author: Kevin Evans
Author: Mo L. Clark
Author: Keith N. Frayn

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