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The effect of low protein diet in pregnancy on the development of brain metabolism in rat offspring

The effect of low protein diet in pregnancy on the development of brain metabolism in rat offspring
The effect of low protein diet in pregnancy on the development of brain metabolism in rat offspring
The effect of maternal low protein diet in pregnancy on the function of offspring cerebral cytochrome c oxidase (CcO) was investigated in vitro immediately before and after birth, using fetal and neonatal rat pup forebrain tissue. Pregnant rat dams were fed either a control (C, 18% casein n = 22) or low protein (LP, 9% casein n = 14) diet. Cerebral tissues were harvested from pups the day before (E21) and after (P1) birth. A Clarke electrode chamber was used to determine O2 consumption in brain tissue homogenate, under baseline conditions with and without the mitochondrial electron transport chain inhibitor myxothiazol and in the presence of incremental doses of the electron donor N',N',N',N'-tetramethyl-p-phenylenediamide (TMPD) with myxothiazol. Maximal stimulated CcO activity {tjp_1146_mu1} was less in LP versus C pups at both E21 (P < 0.001) and P1 (P < 0.05). At E21 only, sensitivity to electron flux (pEC50) was greater (P < 0.001) in LP compared to C offspring. In addition, {tjp_1146_mu2} was reduced and pEC50 was greater after birth (i.e. P1 versus E21) in C (P < 0.001) but not in LP pups. This is the first report of the effects of maternal dietary imbalance in pregnancy on offspring cerebral metabolic function. The effects may form part of a developmental adaptive response to reduce energy consumption and promote perinatal survival, or to confer advantage in a postnatal environment predicted to be nutritionally poor.
0022-3751
553-558
Gallagher, E.A.L.
0496c347-db95-40da-ba0e-4a7b5593bb50
Newman, J.P.
38b6cc7a-a882-40fd-a03a-388aae18416d
Green, L.R.
8a601974-efe5-4916-9268-9e7bc72d89c5
Hanson, M.A.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Gallagher, E.A.L.
0496c347-db95-40da-ba0e-4a7b5593bb50
Newman, J.P.
38b6cc7a-a882-40fd-a03a-388aae18416d
Green, L.R.
8a601974-efe5-4916-9268-9e7bc72d89c5
Hanson, M.A.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f

Gallagher, E.A.L., Newman, J.P., Green, L.R. and Hanson, M.A. (2005) The effect of low protein diet in pregnancy on the development of brain metabolism in rat offspring. Journal of Physiology, 568 (2), 553-558. (doi:10.1113/jphysiol.2005.092825).

Record type: Article

Abstract

The effect of maternal low protein diet in pregnancy on the function of offspring cerebral cytochrome c oxidase (CcO) was investigated in vitro immediately before and after birth, using fetal and neonatal rat pup forebrain tissue. Pregnant rat dams were fed either a control (C, 18% casein n = 22) or low protein (LP, 9% casein n = 14) diet. Cerebral tissues were harvested from pups the day before (E21) and after (P1) birth. A Clarke electrode chamber was used to determine O2 consumption in brain tissue homogenate, under baseline conditions with and without the mitochondrial electron transport chain inhibitor myxothiazol and in the presence of incremental doses of the electron donor N',N',N',N'-tetramethyl-p-phenylenediamide (TMPD) with myxothiazol. Maximal stimulated CcO activity {tjp_1146_mu1} was less in LP versus C pups at both E21 (P < 0.001) and P1 (P < 0.05). At E21 only, sensitivity to electron flux (pEC50) was greater (P < 0.001) in LP compared to C offspring. In addition, {tjp_1146_mu2} was reduced and pEC50 was greater after birth (i.e. P1 versus E21) in C (P < 0.001) but not in LP pups. This is the first report of the effects of maternal dietary imbalance in pregnancy on offspring cerebral metabolic function. The effects may form part of a developmental adaptive response to reduce energy consumption and promote perinatal survival, or to confer advantage in a postnatal environment predicted to be nutritionally poor.

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Published date: 2005
Organisations: Dev Origins of Health & Disease

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Local EPrints ID: 25524
URI: http://eprints.soton.ac.uk/id/eprint/25524
ISSN: 0022-3751
PURE UUID: 636bdcd6-c4fc-427e-b69d-7cbdc77fed15
ORCID for L.R. Green: ORCID iD orcid.org/0000-0001-7423-9696
ORCID for M.A. Hanson: ORCID iD orcid.org/0000-0002-6907-613X

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Date deposited: 10 Apr 2006
Last modified: 16 Mar 2024 03:19

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Contributors

Author: E.A.L. Gallagher
Author: J.P. Newman
Author: L.R. Green ORCID iD
Author: M.A. Hanson ORCID iD

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