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Selective partitioning of dietary fatty acids into the VLDL TG pool in the early postprandial period

Heath, Richard B., Karpe, Fredrik, Milne, Ross W., Burdge, Graham C., Wootton, Stephen A. and Frayn, Keith N. (2003) Selective partitioning of dietary fatty acids into the VLDL TG pool in the early postprandial period Journal of Lipid Research, 44, (11), pp. 2065-2072. (doi:10.1194/jlr.M300167-JLR200).

Record type: Article


Circulating triacylglycerol (TG) arises mainly from dietary fat. However, little is known about the entry of dietary fat into the major TG pool, very low-density lipoprotein (VLDL) TG. We used a novel method to study the specific incorporation of dietary fatty acids into postprandial VLDL TG in humans. Eight healthy volunteers (age 25.4 ± 2.2 years, body mass index 22.1 ± 2.3 kg/m2) were fed a mixed meal containing 30 g fish oil and 600 mg [1-13C]palmitic acid. Chylomicrons and VLDL were separated using immunoaffinity against apolipoprotein B-100. The fatty acid composition of lipoproteins was analyzed by gas chromatography/mass spectrometry. [1-13C]palmitic acid started to appear in VLDL TG 3 h after meal intake, and a similar delay was observed for eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Approximately 20% of dietary fatty acids entered the VLDL TG pool 6 h after meal intake. DHA was clearly overincorporated into this pool compared with [1-13C]palmitic acid and EPA. This seemed to depend on a marked elevation of this fatty acid in the nonesterified fatty acid pool.

In summary, the contribution of dietary fatty acids to early postprandial VLDL TG is substantial. The role of DHA in VLDL TG production will require further investigation.

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Published date: 2003
Keywords: chylomicron, apolipoprotein b, immunoaffinity chromatography, triacylglycerol, n-3 polyunsaturated fatty acids, palmitic acid, stable isotopes


Local EPrints ID: 25604
ISSN: 0022-2275
PURE UUID: bfc874f6-7838-42e2-b29c-256922dfcaff
ORCID for Graham C. Burdge: ORCID iD

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Date deposited: 19 Apr 2006
Last modified: 17 Jul 2017 16:10

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Author: Richard B. Heath
Author: Fredrik Karpe
Author: Ross W. Milne
Author: Keith N. Frayn

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