The University of Southampton
University of Southampton Institutional Repository

Relation between the fatty acid composition of peripheral blood mononuclear cells and measures of immune cell function in healthy, free-living subjects aged 25-72 y1-3

Relation between the fatty acid composition of peripheral blood mononuclear cells and measures of immune cell function in healthy, free-living subjects aged 25-72 y1-3
Relation between the fatty acid composition of peripheral blood mononuclear cells and measures of immune cell function in healthy, free-living subjects aged 25-72 y1-3
Background: There is little information about the relation between the fatty acid composition of human immune cells and the function of those cells over the habitual range of fatty acid intakes.
Objective: The objective of the study was to determine the relation between the fatty acid composition of human peripheral blood mononuclear cell (PBMC) phospholipids and the functions of human immune cells.
Design: One hundred fifty healthy adult subjects provided a fasting blood sample. The phagocytic and oxidative burst activities of monocytes and neutrophils were measured in whole blood. PBMCs were isolated and used to measure lymphocyte proliferation in response to the T cell mitogen concanavalin A and the production of cytokines in response to concanavalin A or bacterial lipopolysaccharide. The fatty acid composition of plasma and PBMC phospholipids was determined.
Results: Wide variations in fatty acid composition of PBMC phospholipids and immune cell functions were identified among the subjects. The proportions of total polyunsaturated fatty acids (PUFAs), of total n-6 and n-3 PUFAs, and of several individual PUFAs in PBMC phospholipids were positively correlated with phagocytosis by neutrophils and monocytes, neutrophil oxidative burst, lymphocyte proliferation, and interferon ? production. The ratios of saturated fatty acids to PUFAs and of n-6 to n-3 PUFAs were negatively correlated with these same immune functions. The relation of PBMC fatty acid composition to monocyte oxidative burst was the reverse of its relation to monocyte phagocytosis and neutrophil oxidative burst.
Conclusion: Variations in the fatty acid composition of PBMC phospholipids account for some of the variability in immune cell functions among healthy adults.
fatty acid, immunity, lymphocytes, monocytes, neutrophils, cytokines, phagocytosis, oxidative burst
0002-9165
1278-1286
Kew, Samantha
0388d70a-7691-4660-9896-95a45099a5db
Banerjee, Tapati
268e92f6-7802-44b4-a916-746c2433fa71
Minihane, Anne M.
323fcab6-215c-4c13-b8d4-2ad242a31900
Finnegan, Yvonne E.
55946247-9cb4-4562-b11b-30e410669aac
Williams, Christine M.
4cf5b7be-8973-4ed3-9150-5caf74999670
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Kew, Samantha
0388d70a-7691-4660-9896-95a45099a5db
Banerjee, Tapati
268e92f6-7802-44b4-a916-746c2433fa71
Minihane, Anne M.
323fcab6-215c-4c13-b8d4-2ad242a31900
Finnegan, Yvonne E.
55946247-9cb4-4562-b11b-30e410669aac
Williams, Christine M.
4cf5b7be-8973-4ed3-9150-5caf74999670
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6

Kew, Samantha, Banerjee, Tapati, Minihane, Anne M., Finnegan, Yvonne E., Williams, Christine M. and Calder, Philip C. (2003) Relation between the fatty acid composition of peripheral blood mononuclear cells and measures of immune cell function in healthy, free-living subjects aged 25-72 y1-3. American Journal of Clinical Nutrition, 77 (5), 1278-1286.

Record type: Article

Abstract

Background: There is little information about the relation between the fatty acid composition of human immune cells and the function of those cells over the habitual range of fatty acid intakes.
Objective: The objective of the study was to determine the relation between the fatty acid composition of human peripheral blood mononuclear cell (PBMC) phospholipids and the functions of human immune cells.
Design: One hundred fifty healthy adult subjects provided a fasting blood sample. The phagocytic and oxidative burst activities of monocytes and neutrophils were measured in whole blood. PBMCs were isolated and used to measure lymphocyte proliferation in response to the T cell mitogen concanavalin A and the production of cytokines in response to concanavalin A or bacterial lipopolysaccharide. The fatty acid composition of plasma and PBMC phospholipids was determined.
Results: Wide variations in fatty acid composition of PBMC phospholipids and immune cell functions were identified among the subjects. The proportions of total polyunsaturated fatty acids (PUFAs), of total n-6 and n-3 PUFAs, and of several individual PUFAs in PBMC phospholipids were positively correlated with phagocytosis by neutrophils and monocytes, neutrophil oxidative burst, lymphocyte proliferation, and interferon ? production. The ratios of saturated fatty acids to PUFAs and of n-6 to n-3 PUFAs were negatively correlated with these same immune functions. The relation of PBMC fatty acid composition to monocyte oxidative burst was the reverse of its relation to monocyte phagocytosis and neutrophil oxidative burst.
Conclusion: Variations in the fatty acid composition of PBMC phospholipids account for some of the variability in immune cell functions among healthy adults.

This record has no associated files available for download.

More information

Published date: 2003
Keywords: fatty acid, immunity, lymphocytes, monocytes, neutrophils, cytokines, phagocytosis, oxidative burst

Identifiers

Local EPrints ID: 25715
URI: http://eprints.soton.ac.uk/id/eprint/25715
ISSN: 0002-9165
PURE UUID: 619de491-5822-48b6-97d6-1c8724d5c234
ORCID for Philip C. Calder: ORCID iD orcid.org/0000-0002-6038-710X

Catalogue record

Date deposited: 21 Apr 2006
Last modified: 23 Jul 2022 01:39

Export record

Contributors

Author: Samantha Kew
Author: Tapati Banerjee
Author: Anne M. Minihane
Author: Yvonne E. Finnegan
Author: Christine M. Williams

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×