?-tocopherol concentrations, lipid peroxidation and superoxide dismutase and glutathione peroxidase activities in rat heart and liver after feeding stabilized and unstabilized fish oil
?-tocopherol concentrations, lipid peroxidation and superoxide dismutase and glutathione peroxidase activities in rat heart and liver after feeding stabilized and unstabilized fish oil
To further examine the relationship between increased consumption of n-3 polyunsaturated fatty acids (PUFAs), antioxidant defence systems and lipid peroxidation, for 6 weeks adult male rats were fed fish oil (FO)-rich diets containing one of two levels of ?-tocopherol (4.5 or 1.9 IU vitamin E/g fish oil) either with or without the addition of the antioxidant mix PUFANOX(R) in order to stabilize the FO; rats fed corn oil or butter served as controls.
Feeding FO resulted in increased proportions of the n-3 PUFAs eicosapentaenoic and docosahexaenoic acids in the phospholipids of the plasma, myocardium and liver. Rats fed the PUFANOX(R)-stabilized FO had higher plasma, myocardial and liver ?-tocopherol concentrations compared to those fed the unstabilized FO; -tocopherol concentrations were highest in rats fed the higher level of -tocopherol in combination with PUFANOX(R). FO feeding increased lipid peroxidation in myocardial and liver extracts. This was highest after feeding the FO diet which contained the lower amount of ?-tocopherol and no PUFANOX(R) and was positively correlated with the n-3 PUFA content of the phospholipids. FO feeding did not alter myocardial and liver superoxide dismutase activity. Myocardial glutathione peroxidase activity was lower after feeding FO, except that containing the higher amount of -tocopherol plus PUFANOX(R). Glutathione peroxidase activity in the liver was lower after FO feeding than after corn oil with no significant differences among the different FO diets. Thus, insufficient antioxidant protection in FO results in decreased antioxidant defences and increased lipid peroxidation. Increasing the content of ?-tocopherol and including a stabilizing agent (e.g. PUFANOX(R)) in FO can prevent at least some of these effects. The results of the present study do not support the idea that intake of FO, by increasing tissue lipid peroxidation, induces the body's antioxidant defence system.
fish oil, polyunsaturated fatty acid, antioxidant, ?-tocopherol, oxidative stress
1529-1544
Luostarinen, R.L.
22084232-1d75-441d-b99d-5bc2887ecd12
Laasonen, K.
adb26478-ccac-4829-9cee-89c4f2b96adf
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
2001
Luostarinen, R.L.
22084232-1d75-441d-b99d-5bc2887ecd12
Laasonen, K.
adb26478-ccac-4829-9cee-89c4f2b96adf
Calder, P.C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Luostarinen, R.L., Laasonen, K. and Calder, P.C.
(2001)
?-tocopherol concentrations, lipid peroxidation and superoxide dismutase and glutathione peroxidase activities in rat heart and liver after feeding stabilized and unstabilized fish oil.
Nutrition Research, 21 (12), .
(doi:10.1016/S0271-5317(01)00387-6).
Abstract
To further examine the relationship between increased consumption of n-3 polyunsaturated fatty acids (PUFAs), antioxidant defence systems and lipid peroxidation, for 6 weeks adult male rats were fed fish oil (FO)-rich diets containing one of two levels of ?-tocopherol (4.5 or 1.9 IU vitamin E/g fish oil) either with or without the addition of the antioxidant mix PUFANOX(R) in order to stabilize the FO; rats fed corn oil or butter served as controls.
Feeding FO resulted in increased proportions of the n-3 PUFAs eicosapentaenoic and docosahexaenoic acids in the phospholipids of the plasma, myocardium and liver. Rats fed the PUFANOX(R)-stabilized FO had higher plasma, myocardial and liver ?-tocopherol concentrations compared to those fed the unstabilized FO; -tocopherol concentrations were highest in rats fed the higher level of -tocopherol in combination with PUFANOX(R). FO feeding increased lipid peroxidation in myocardial and liver extracts. This was highest after feeding the FO diet which contained the lower amount of ?-tocopherol and no PUFANOX(R) and was positively correlated with the n-3 PUFA content of the phospholipids. FO feeding did not alter myocardial and liver superoxide dismutase activity. Myocardial glutathione peroxidase activity was lower after feeding FO, except that containing the higher amount of -tocopherol plus PUFANOX(R). Glutathione peroxidase activity in the liver was lower after FO feeding than after corn oil with no significant differences among the different FO diets. Thus, insufficient antioxidant protection in FO results in decreased antioxidant defences and increased lipid peroxidation. Increasing the content of ?-tocopherol and including a stabilizing agent (e.g. PUFANOX(R)) in FO can prevent at least some of these effects. The results of the present study do not support the idea that intake of FO, by increasing tissue lipid peroxidation, induces the body's antioxidant defence system.
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Published date: 2001
Keywords:
fish oil, polyunsaturated fatty acid, antioxidant, ?-tocopherol, oxidative stress
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Local EPrints ID: 25774
URI: http://eprints.soton.ac.uk/id/eprint/25774
ISSN: 0271-5317
PURE UUID: 412f19c4-fff6-49b4-944b-edfb731fb605
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Date deposited: 21 Apr 2006
Last modified: 16 Mar 2024 02:51
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Author:
R.L. Luostarinen
Author:
K. Laasonen
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